DNA binding by the coliphage 186 repressor protein C1

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dc.contributor.authorDodd, I.
dc.contributor.authorEgan, J.
dc.date.issued1996
dc.description.abstractThe cI gene of coliphage 186 maintains lysogeny and confers immunity to 186 infection by repressing the major early promoter, p(R), and the promoter for the late transcription activator gene, p(B). Gel mobility shirt and DNase I footprinting show that CI protein binds to the DNA at p(R) and p(B) and also to sites approximately 300 base pairs upstream and downstream of p(R), called FL and FR. Mutations which cause virulence reduce CI binding to p(R). The biochemical and genetic data identify three CI operators at p(R), two at p(B), and single operators at FL and FR. The operators at the p(B), FL, FR, and central p(R) sites are inverted repeat sequences, separated by 5 base pairs (Type A) or, in the case of p(R), by 4 base pairs (Type A'). A different inverted repeat operator sequence (Type B) is proposed for the binding sites on each side of the central site at p(R). Thus, CI appears to recognize two distinct DNA sequences. CI binds cooperatively to adjacent operators, and binding at p(R) is strongly dependent on these cooperative interactions. A high order CI multimer appears to be the active DNA binding species, even at single operators.
dc.identifier.citationJournal of Biological Chemistry, 1996; 271(19):11532-11540
dc.identifier.doi10.1074/jbc.271.19.11532
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.orcidDodd, I. [0000-0003-2969-6841]
dc.identifier.urihttp://hdl.handle.net/2440/11315
dc.language.isoen
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.source.urihttps://doi.org/10.1074/jbc.271.19.11532
dc.subjectEscherichia coli
dc.subjectColiphages
dc.subjectDeoxyribonuclease I
dc.subjectDNA-Binding Proteins
dc.subjectRepressor Proteins
dc.subjectViral Proteins
dc.subjectDNA Primers
dc.subjectDNA Footprinting
dc.subjectPolymerase Chain Reaction
dc.subjectLysogeny
dc.subjectBase Composition
dc.subjectBinding Sites
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectRepetitive Sequences, Nucleic Acid
dc.subjectConsensus Sequence
dc.subjectKinetics
dc.subjectGenes, Fungal
dc.subjectPlasmids
dc.subjectMolecular Sequence Data
dc.subjectViral Regulatory and Accessory Proteins
dc.subjectPromoter Regions, Genetic
dc.titleDNA binding by the coliphage 186 repressor protein C1
dc.typeJournal article
pubs.publication-statusPublished

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