Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis

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dc.contributor.authorLokman, N.
dc.contributor.authorElder, A.
dc.contributor.authorRicciardelli, C.
dc.contributor.authorOehler, M.
dc.date.issued2012
dc.description.abstractThe majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the in vivo assessment of potential new therapeutic targets in ovarian cancer. However, animal models are costly and time consuming. Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM assays have been widely used to study angiogenesis and tumor invasion of colorectal, prostate and brain cancers. However, there have been limited studies that have used CAM assays to assess ovarian cancer invasion and metastasis. We have therefore developed a CAM assay protocol to monitor the metastatic properties of ovarian cancer cells (OVCAR-3, SKOV-3 and OV-90) and to study the effect of potential therapeutic molecules in vivo. The results from the CAM assay are consistent with cancer cell motility and invasion observed in in vitro assays. Our results demonstrate that the CAM assay is a robust and cost effective model to study ovarian cancer cell metastasis. It is therefore a very useful in vivo model for screening of potential novel therapeutics.
dc.description.statementofresponsibilityNoor A. Lokman, Alison S.F. Elder, Carmela Ricciardelli and Martin K. Oehler
dc.identifier.citationInternational Journal of Molecular Sciences, 2012; 13(8):9959-9970
dc.identifier.doi10.3390/ijms13089959
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.orcidLokman, N. [0000-0002-2071-5308]
dc.identifier.orcidRicciardelli, C. [0000-0001-7415-1854]
dc.identifier.orcidOehler, M. [0000-0002-2651-5913]
dc.identifier.urihttp://hdl.handle.net/2440/73656
dc.language.isoen
dc.publisherMDPI AG
dc.rights© 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
dc.source.urihttps://doi.org/10.3390/ijms13089959
dc.subjectOvarian cancer
dc.subjectinvasion
dc.subjectmetastasis
dc.subjectchick chorioallantoic membrane
dc.titleChick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis
dc.typeJournal article
pubs.publication-statusPublished

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