Spinal motor axons and neural crest cells use different molecular guides for segmental migration through the rostral half-somite

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dc.contributor.authorKoblar, S.
dc.contributor.authorKrull, C.
dc.contributor.authorPasquale, E.
dc.contributor.authorMcLennan, R.
dc.contributor.authorPeale, F.
dc.contributor.authorCerretti, D.
dc.contributor.authorBothwell, M.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2000
dc.descriptionThe definitive version may be found at www.wiley.com
dc.description.abstractThe peripheral nervous system in vertebrates is composed of repeating metameric units of spinal nerves. During development, factors differentially expressed in a rostrocaudal pattern in the somites confine the movement of spinal motor axons and neural crest cells to the rostral half of the somitic sclerotome. The expression patterns of transmembrane ephrin-B ligands and interacting EphB receptors suggest that these proteins are likely candidates for coordinating the segmentation of spinal motor axons and neural crest cells. In vitro, ephrin-B1 has indeed been shown to repel axons extending from the rodent neural tube (Wang & Anderson, 1997). In avians, blocking interactions between EphB3 expressed by neural crest cells and ephrin-B1 localized to the caudal half of the somite in vivo resulted in loss of the rostrocaudal patterning of trunk neural crest migration (Krull et al., 1997). The role of ephrin-B1 in patterning spinal motor axon outgrowth in avian embryos was investigated. Ephrin-B1 protein was found to be expressed in the caudal half-sclerotome and in the dermomyotome at the appropriate time to interact with the EphB2 receptor expressed on spinal motor axons. Treatment of avian embryo explants with soluble ephrin-B1, however, did not perturb the segmental outgrowth of spinal motor axons through the rostral half-somite. In contrast, under the same treatment conditions with soluble ephrin-B1, neural crest cells migrated aberrantly through both rostral and caudal somite halves. These results indicate that the interaction between ephrin-B1 and EphB2 is not required for patterning spinal motor axon segmentation. Even though spinal motor axons traverse the same somitic pathway as neural crest cells, different molecular guidance mechanisms appear to influence their movement.
dc.identifier.citationDevelopmental Neurobiology, 2000; 43(4):437-447
dc.identifier.doi10.1002/(SICI)1097-4695(200003)42:4<437::AID-NEU5>3.0.CO;2-O
dc.identifier.issn1932-8451
dc.identifier.issn0022-3034
dc.identifier.orcidKoblar, S. [0000-0002-8667-203X]
dc.identifier.urihttp://hdl.handle.net/2440/28018
dc.language.isoen
dc.publisherJohn Wiley & Sons Inc
dc.source.urihttp://www3.interscience.wiley.com/journal/70002870/abstract
dc.subjectSpinal Cord
dc.subjectAxons
dc.subjectMotor Neurons
dc.subjectChick Embryo
dc.subjectSomites
dc.subjectNeural Crest
dc.subjectAnimals
dc.subjectHumans
dc.subjectEphrin-B1
dc.subjectEphrin-B2
dc.subjectMembrane Proteins
dc.subjectRecombinant Fusion Proteins
dc.subjectRNA, Messenger
dc.subjectCulture Techniques
dc.subjectImmunohistochemistry
dc.subjectCell Movement
dc.subjectBody Patterning
dc.subjectImmunoglobulin Fc Fragments
dc.titleSpinal motor axons and neural crest cells use different molecular guides for segmental migration through the rostral half-somite
dc.typeJournal article
pubs.publication-statusPublished

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