Structural organization of the mouse and human GALR1 Galanin receptor genes (Galnr and GALNR) and chromosomal localization of the mouse gene

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dc.contributor.authorJacoby, A.
dc.contributor.authorWebb, G.
dc.contributor.authorLiu, M.
dc.contributor.authorKofler, B.
dc.contributor.authorHort, Y.
dc.contributor.authorFathi, Z.
dc.contributor.authorBottema, C.
dc.contributor.authorShine, J.
dc.contributor.authorIismaa, T.
dc.date.issued1997
dc.description.abstractThe neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Human and rat GALR1 galanin receptor cDNA clones have previously been isolated using expression cloning. We have used the human GALR1 cDNA in hybridization screening to isolate the gene encoding GALR1 in both human (GALNR) and mouse (Galnr). The gene spans approximately 15-20 kb in both species; its structural organization is conserved and is unique among G-protein-coupled receptors. The coding sequence is contained on three exons, with exon 1 encoding the N-terminal end of the receptor and the first five transmembrane domains. Exon 2 encodes the third intracellular loop, while exon 3 encodes the remainder of the receptor, from transmembrane domain 6 to the C-terminus of the receptor protein. The mouse and human GALR1 receptor proteins are 348 and 349 amino acids long, respectively, and display 93% identity at the amino acid level. The mouse Galnr gene has been localized to Chromosome 18E4, homoeologous with the previously reported localization of the human GALNR gene to 18q23 in the same syntenic group as the genes encoding nuclear factor of activated T-cells, cytoplasmic 1, and myelin basic protein.
dc.description.statementofresponsibilityJacoby, Arie S. ; Webb, Graham C. ; Liu, Marjorie L. ; Kofler, Barbara ; Hort, Yvonne J. ; Fathi, Zahra ; Bottema, Cynthia D.K. ; Shine, John ; Iismaa, Tiina P.
dc.identifier.citationGenomics, 1997; 45(3):496-508
dc.identifier.doi10.1006/geno.1997.4960
dc.identifier.issn0888-7543
dc.identifier.issn1089-8646
dc.identifier.orcidBottema, C. [0000-0001-6245-0099]
dc.identifier.urihttp://hdl.handle.net/2440/13234
dc.language.isoen
dc.publisherACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
dc.source.urihttps://doi.org/10.1006/geno.1997.4960
dc.subjectAnimals
dc.subjectMice, Inbred BALB C
dc.subjectMice, Inbred C57BL
dc.subjectHumans
dc.subjectMice
dc.subjectReceptors, Galanin
dc.subjectReceptors, Gastrointestinal Hormone
dc.subjectDNA, Complementary
dc.subjectChromosome Mapping
dc.subjectCloning, Molecular
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectConserved Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectMolecular Sequence Data
dc.subjectMale
dc.titleStructural organization of the mouse and human GALR1 Galanin receptor genes (Galnr and GALNR) and chromosomal localization of the mouse gene
dc.typeJournal article
pubs.publication-statusPublished

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