Studies on (1→3)-[β]-glucan synthases in barley / by Jing Li.
Date
2003
Authors
Li, Jing
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Thesis
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Abstract
A putative callose synthase gene, designated HvGSL1 cDNA is siolated from barley and its involvement in callose biosynthesis is investigated. A near-full length HvGSL1 cDNA encoded a protein showing approximately 30% identitly with that of yeast FKS genes at the amino acid level. The function of this geen was investigated by heterologous expression, protein purification, immunochemistry and mass spectrometric analysis. Results provide strong evidence that the gene encodes a protein which is associated with callose synthase activity, and is likely to encode the catalytic subunit of the synthase complex.
School/Discipline
School of Agriculture, Food and Wine : Plant and Pest Science
Dissertation Note
Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Discipline of Plant and Pest Science, 2003
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Description
Bibliography: leaves 132-155.
viii, 155 leaves : ill., plates, charts (some col.) ; 30 cm.
viii, 155 leaves : ill., plates, charts (some col.) ; 30 cm.