Isolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii comple

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dc.contributor.authorGardner, M.
dc.contributor.authorOttewell, K.
dc.contributor.authorAdams, M.
dc.date.issued2004
dc.descriptionThe definitive version is available at www.blackwell-synergy.com Article first published online: 24 FEB 2004
dc.description.abstractTetra and tri-nucleotide microsatellite loci were isolated from a parthenogenetic form of the Australian lizard Menetia greyii, using an enrichment method with polymerase chain reaction (PCR)-based colony screening. Primers for 23 loci were designed and 11 of these loci amplified well in a panel of 10 parthenogenetic individuals from a single population. Seven loci were further characterized and six successfully amplified and were polymorphic in all five sexual species of the Menetia greyii complex they were tested in. These loci will be used to investigate the parental ancestry and clonal diversity of various parthenogenetic forms within the Menetia greyii species complex.
dc.description.statementofresponsibilityM. G. Gardner, K. Ottewell, M. Adams
dc.identifier.citationMolecular Ecology Resources, 2004; 4(2):219-221
dc.identifier.doi10.1111/j.1471-8286.2004.00622
dc.identifier.issn1755-098X
dc.identifier.orcidAdams, M. [0000-0002-6010-7382]
dc.identifier.urihttp://hdl.handle.net/2440/44736
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.rights© 2004 Blackwell Publishing Ltd
dc.subjectenrichment
dc.subjectMenetia greyii
dc.subjectmicrosatellite
dc.subjectparthenogenesis
dc.subjectskink
dc.subjectunisexual
dc.titleIsolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii comple
dc.typeJournal article
pubs.publication-statusPublished

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