Circulating insulin-like growth factors-I and -II and substrates in fetal sheep following restriction of placental growth

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dc.contributor.authorOwens, J.A.
dc.contributor.authorKind, K.L.
dc.contributor.authorCarbone, F.
dc.contributor.authorRobinson, J.S.
dc.contributor.authorOwens, P.C.
dc.date.issued1994
dc.description.abstractTo determine the relationship between placental delivery of oxygen and glucose, circulating insulin-like growth factors (IGFs) and fetal growth, the effect of variable restriction of placental growth was determined in sheep in late gestation. Arterial blood was obtained via indwelling catheters at 120 and 127 days of gestation, prior to necropsy at 130 days to measure fetal and placental weights. Plasma was acidified and subjected to size-exclusion high-performance liquid chromatography at pH 2.8 to dissociate and separate IGFs from their binding proteins. The acid-dissociated IGF fraction was analysed by sensitive and highly specific radioligand assays for IGF-I and IGF-II, previously defined using ovine IGFs. Fetal weight and blood pO2 and glucose at 120 and 127 days of gestation correlated positively with placental weight. Plasma IGF-I was positively associated with fetal weight and fetal liver weight, and with blood pO2 and glucose at both ages. Plasma IGF-II levels also correlated positively with fetal weight, fetal liver weight and with blood glucose and pO2, but only at 127 days of gestation. In the most severely growth-retarded fetal sheep, blood glucose and pO2 and plasma IGF-I were significantly reduced when compared with normal fetuses at 120 days. All decreased further by 127 days of gestation as did plasma IGF-II in severely growth-retarded fetal sheep compared with normal fetuses. These observations are consistent with the hypothesis that both IGF-I and IGF-II are chronically regulated by oxygen and nutrition in utero and mediate part of the influence of placental supply of substrate over fetal growth.
dc.description.statementofresponsibilityJ A Owens, K L Kind, F Carbone, J S Robinson and P C Owens
dc.identifier.citationJournal of Endocrinology, 1994; 140(1):5-13
dc.identifier.doi10.1677/joe.0.1400005
dc.identifier.issn0022-0795
dc.identifier.issn1479-6805
dc.identifier.orcidOwens, J.A. [0000-0002-7498-1353]
dc.identifier.orcidRobinson, J.S. [0000-0002-4515-6039]
dc.identifier.urihttp://hdl.handle.net/2440/86770
dc.language.isoen
dc.publisherBioScientifica
dc.rights© 1994 Journal of Endocrinology Ltd
dc.source.urihttps://doi.org/10.1677/joe.0.1400005
dc.subjectLiver
dc.subjectFetal Blood
dc.subjectAnimals
dc.subjectSheep
dc.subjectHumans
dc.subjectOxygen
dc.subjectBlood Glucose
dc.subjectSomatomedins
dc.subjectInsulin-Like Growth Factor I
dc.subjectInsulin-Like Growth Factor II
dc.subjectRadioligand Assay
dc.subjectOrgan Size
dc.subjectChromatography, High Pressure Liquid
dc.subjectEmbryonic and Fetal Development
dc.subjectGestational Age
dc.subjectPregnancy
dc.subjectPlacentation
dc.subjectFemale
dc.titleCirculating insulin-like growth factors-I and -II and substrates in fetal sheep following restriction of placental growth
dc.typeJournal article
pubs.publication-statusPublished

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