Construction of a high-resolution physical and transcription map of chromosome 16q24.3 - a region of frequent loss of heterozygosity in sporadic breast cancer

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dc.contributor.authorWhitmore, S.
dc.contributor.authorCrawford, J.
dc.contributor.authorApostolou, S.
dc.contributor.authorEyre, H.
dc.contributor.authorBaker, E.
dc.contributor.authorLower, K.
dc.contributor.authorSettasatian, C.
dc.contributor.authorGoldup, S.
dc.contributor.authorSeshadri, R.
dc.contributor.authorGibson, R.
dc.contributor.authorMathew, C.
dc.contributor.authorCleton-Jansen, A.M.
dc.contributor.authorSavoia, A.
dc.contributor.authorPronk, J.
dc.contributor.authorAuerbach, A.
dc.contributor.authorDoggett, N.
dc.contributor.authorSutherland, G.
dc.contributor.authorCallen, D.
dc.date.issued1998
dc.description.abstractA breast cancer tumor suppressor gene has been localized to chromosome 16q24.3 by loss of heterozygosity (LOH) studies of breast tumor DNA. To identify candidate genes for this suppressor function, we have constructed a detailed physical map extending approximately 940 kb from the telomere of the long arm of chromosome 16 that encompasses the minimum LOH interval. This contig consists of a minimum overlapping set of 35 cosmids and a single PAC clone that were aligned by restriction enzyme site mapping. Cosmids were initially identified by screening filters with markers localized to the region by physical mapping using mouse/human somatic cell hybrids, and subsequently cosmid ends were used to complete the contig. A total of seven known genes, including PRSM1, PISSLRE, and the recently cloned Fanconi anemia A (FAA) gene, and potential transcripts from exon-trapping experiments have been located to this contig. A minimum of 14 new transcripts have been identified based on homology of trapped exons with database sequences. This contig and expressed sequence map will form the basis for the identification of the breast cancer tumor suppressor gene in this region.
dc.description.statementofresponsibilityScott A. Whitmore, Joanna Crawford, Sinoula Apostolou, Helen Eyre, Elizabeth Baker, Karen M. Lower, Chatri Settasatian, Sandra Goldup, Ram Seshadri, Rachel A. Gibson, Christopher G. Mathew, Anne-Marie Cleton-Jansen, Anna Savoia, Jan C. Pronk, Arleen D. Auerbach, Norman A. Doggett, Grant R. Sutherland, David F. Callen
dc.identifier.citationGenomics, 1998; 50(1):1-8
dc.identifier.doi10.1006/geno.1998.5316
dc.identifier.issn0888-7543
dc.identifier.issn1089-8646
dc.identifier.orcidCallen, D. [0000-0002-6189-9991]
dc.identifier.urihttp://hdl.handle.net/2440/7280
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.rightsCopyright © 1998 Academic Press. All rights reserved.
dc.source.urihttps://doi.org/10.1006/geno.1998.5316
dc.subjectChromosomes, Human, Pair 16
dc.subjectHumans
dc.subjectBreast Neoplasms
dc.subjectGenetic Markers
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectPhysical Chromosome Mapping
dc.subjectTranscription, Genetic
dc.subjectLoss of Heterozygosity
dc.subjectExons
dc.subjectMolecular Sequence Data
dc.titleConstruction of a high-resolution physical and transcription map of chromosome 16q24.3 - a region of frequent loss of heterozygosity in sporadic breast cancer
dc.typeJournal article
pubs.publication-statusPublished

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