An infrared spectroscopic study of fucoidan/chitosan polyelectrolyte multilayers for biomedical applications /
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(Published version)
Date
2019
Authors
Benbow, Natalie L.
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Type:
thesis
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Abstract
This thesis explored the build-up of polyelectrolyte multilayers (PEM) composed of fucoidan and chitosan, and the interactions of these PEMs with proteins of relevance for physiological applications, primarily using infrared spectroscopy. The adsorption of bovine serum albumin (BSA), lysozyme (LYZ) and fibroblast growth factor-2 (FGF-2) was investigated, and an in depth study of the degree of hydrogen bonding of water within the films was performed. It was found that the molecular weight of the fucoidan within the film impacted the film structure and BSA adsorption. LYZ was able to penetrate into films built using pharmaceutical grade fucoidan whereas FGF-2 could not penetrate into the same PEMs. The interactions of the proteins with the films had different impacts on the properties of the PEMs i.e. changing the degree of hydration or causing loss of the polysaccharides from the PEMs.
School/Discipline
University of South Australia. Future industries Institute.
Future Industries Institute.
Future Industries Institute.
Dissertation Note
Thesis (PhD(Biomaterials Engineering and Nanomedicine))--University of South Australia, 2019.
Provenance
Copyright 2019 Natalie L. Benbow.
Description
1 ethesis (xxx, 290 pages) :
illustrations (some colour), colour charts.
Includes bibliographical references.
illustrations (some colour), colour charts.
Includes bibliographical references.
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506 0#$fstar $2Unrestricted online access