Receptor activator NF kB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis

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dc.contributor.authorCrotti, T.
dc.contributor.authorSmith, M.
dc.contributor.authorHirsch, R.
dc.contributor.authorSoukoulis, S.
dc.contributor.authorWeedon, H.
dc.contributor.authorCapone, M.
dc.contributor.authorAhern, M.
dc.contributor.authorHaynes, D.
dc.date.issued2003
dc.descriptionThe definitive version is available at www.blackwell-synergy.com Article first published online: 26 JUN 2003
dc.description.abstractObjectives and background: This study investigated the expression of key mediators that regulate differentiation of osteoclasts, receptor activator of nuclear factor κB ligand (RANKL), and its natural inhibitor, osteoprotegerin (OPG), in periodontitis. We aimed to compare the levels of the RANKL and OPG in the granulomatous tissue adjacent to areas of alveolar bone loss from patients with periodontitis to that present in tissue from patients without periodontitis. In addition, we aimed to determine the types of cells expressing these factors in these tissues and to demonstrate the expression of the osteoclastic markers, RANK and tartrate-resistant acid phosphatase (TRAP), in periodontitis. Materials and methods: Frozen biopsy specimens were analysed using specific monoclonal antibodies and were evaluated by semiquantitative analysis and digital image analysis to compare levels of RANKL and OPG protein expression. Double labelling of frozen sections with antibodies to different cell lineage specific markers was used to determine the types of cells expressing these proteins. In situ hybridization was used to detect cells expressing RANK mRNA. Results: Semiquantitative image analysis demonstrated that significantly higher levels of RANKL protein (P < 0.05) were expressed in the periodontitis tissue. Conversely, OPG protein was significantly lower (P < 0.05) in the periodontitis tissues. RANKL protein was associated with lymphocytes and macrophages. OPG protein was associated with endothelial cells in both tissues. Many leukocytes expressing RANK mRNA and TRAP were observed in periodontitis tissues. Conclusion: The change in the levels of these key regulators of osteoclast differentiation may play a major role in the bone loss seen in periodontitis.
dc.description.statementofresponsibilityTania Crotti, Malcolm D. Smith, Robert Hirsch, Steven Soukoulis, Helen Weedon, Maria Capone, Michael J. Ahern, David Haynes
dc.identifier.citationJournal of Periodontal Research, 2003; 38(4):380-387
dc.identifier.doi10.1034/j.1600-0765.2003.00615
dc.identifier.issn0022-3484
dc.identifier.issn1600-0765
dc.identifier.orcidCrotti, T. [0000-0002-5422-3758]
dc.identifier.urihttp://hdl.handle.net/2440/3974
dc.language.isoen
dc.publisherMunksgaard Int Publ Ltd
dc.rightsCopyright © Blackwell Munksgaard Ltd
dc.source.urihttp://www.blackwell-synergy.com/doi/abs/10.1034/j.1600-0765.2003.00615
dc.subjectEndothelium, Vascular
dc.subjectLeukocytes, Mononuclear
dc.subjectOsteoclasts
dc.subjectHumans
dc.subjectAlveolar Bone Loss
dc.subjectPeriodontitis
dc.subjectAcid Phosphatase
dc.subjectIsoenzymes
dc.subjectGlycoproteins
dc.subjectCarrier Proteins
dc.subjectNF-kappa B
dc.subjectMembrane Glycoproteins
dc.subjectReceptors, Tumor Necrosis Factor
dc.subjectReceptors, Cytoplasmic and Nuclear
dc.subjectLigands
dc.subjectCell Differentiation
dc.subjectGene Expression Regulation
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectMiddle Aged
dc.subjectFemale
dc.subjectMale
dc.subjectRANK Ligand
dc.subjectReceptor Activator of Nuclear Factor-kappa B
dc.subjectOsteoprotegerin
dc.subjectBiomarkers
dc.subjectTartrate-Resistant Acid Phosphatase
dc.titleReceptor activator NF kB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis
dc.typeJournal article
pubs.publication-statusPublished

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