Mucosal vaccination with a multicomponent adenovirus-vectored vaccine protects against Streptococcus pneumoniae infection in the lung

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dc.contributor.authorArevalo, M.
dc.contributor.authorXu, Q.
dc.contributor.authorPaton, J.
dc.contributor.authorHollingshead, S.
dc.contributor.authorPichichero, M.
dc.contributor.authorBriles, D.
dc.contributor.authorGirgis, N.
dc.contributor.authorZeng, M.
dc.date.issued2009
dc.description.abstractStreptococcus pneumoniae is a major bacterial respiratory pathogen. Current licensed pneumococcal polysaccharide and polysaccharide–protein conjugate vaccines are administered by an intramuscular injection. In order to develop a new-generation vaccine that can be administered in a needle-free mucosal manner, we have constructed early 1 and 3 gene regions (E1/E3) deleted, replication-defective adenoviral vectors encoding pneumococcal surface antigen A (PsaA), the N-fragment of pneumococcal surface protein A (N-PspA), and the detoxified mutant pneumolysin (PdB) from S. pneumoniae strain D39. Intranasal vaccination with the three adenoviral vectors (Ad/PsaA, Ad/N-PspA, and Ad/PdB) in mice resulted in robust antigen-specific serum immunoglobulin G responses, as demonstrated by an enzyme-linked immunosorbent assay. In addition, nasal mucosal vaccination with the combination of the three adenoviral vectors conferred protection against S. pneumoniae strain D39 colonization in mouse lungs. Taken together, these data demonstrate the feasibility of developing a mucosal vaccine against S. pneumoniae using recombinant adenoviruses for antigen delivery.
dc.description.statementofresponsibilityMaria T. Arévalo, Qingfu Xu, James C. Paton, Susan K. Hollingshead, Michael E. Pichichero, David E. Briles, Natasha Girgis & Mingtao Zeng
dc.identifier.citationFEMS Immunology and Medical Microbiology, 2009; 55(3):346-351
dc.identifier.doi10.1111/j.1574-695X.2008.00518.x
dc.identifier.issn0928-8244
dc.identifier.issn1574-695X
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]
dc.identifier.urihttp://hdl.handle.net/2440/52649
dc.language.isoen
dc.publisherElsevier Science BV
dc.source.urihttps://doi.org/10.1111/j.1574-695x.2008.00518.x
dc.subjectmucosal vaccination
dc.subjectpneumococcal vaccine
dc.subjectprotective immunity
dc.subjectreplication-defective adenovirus
dc.subjectStreptococcus pneumoniae.
dc.titleMucosal vaccination with a multicomponent adenovirus-vectored vaccine protects against Streptococcus pneumoniae infection in the lung
dc.typeJournal article
pubs.publication-statusPublished

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