Insertional Mutagenesis: lessons from Arabidopsis, gaining experience in rice
Date
2005
Authors
Guiderdoni, Emmanuel
Piffanelli, Pietro
Sallaud, Christophe
Bourgeois, Emmanuelle
Johnson, Alexander Arthur Theodore
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Book chapter
Citation
Proceedings of the International Congress "In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution", 27-31 May, 2003 / Tuberosa R., Phillips R.L., Gale M. (eds.): pp.379-402
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Abstract
Insertional mutagenesis is a means of analyzing the function of genes through their
disruption by foreign DNA followed by observation of the effect on phenotype. Insertion
libraries are a key tool in plant functional genomics projects aimed at deciphering
major developmental and physiological processes because they permit both the
discovery of new genes through forward genetics screens and gene detection systems as
well as the validation of genes of known sequence through reverse genetics strategies.
Benefiting from experience accumulated over the last decade in the model dicotyledonous
species Arabidopsis, large insertion line libraries in turn have been generated in
rice (Oryza sativa L.), the model plant for cereals and grasses, in the last five years. The
use of insertion mutagens has varied and encompasses T-DNA, delivered during
co-culture with Agrobacterium, the genetically engineered maize transposable Ac/Ds
system and the endogenous ty1-copia retroelement Tos17, mobilized during tissue culture.
Here we review the current status of rice insertional mutagenesis by concentrating
on specific features and advantages and drawbacks of each mutagen, and examine
the establishment of this new resource from a rice functional genomics perspective.