Transthyretin interacts with the lysosome-associated membrane protein (LAMP-1) in circulation

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dc.contributor.authorChang, M.
dc.contributor.authorHua, C.
dc.contributor.authorIsaac, E.
dc.contributor.authorLitjens, T.
dc.contributor.authorHodge, G.
dc.contributor.authorKarageorgos, L.
dc.contributor.authorMeikle, P.
dc.date.issued2004
dc.description.abstractLAMP-1 (lysosome-associated membrane protein), a major glycoprotein present in the lysosomal membrane, constitutes up to 50% of total membrane proteins. LAMP-1, expressed at the plasma membrane, is reported to be the major molecule expressing the sialyl-Lewis X antigen. Two forms of LAMP-1 exist; the full-length LAMP-1 [LAMP-1 (+Tail)] has a highly glycosylated lumenal domain, a membrane-spanning domain and a short cytoplasmic tail, and the truncated LAMP-1 [LAMP-1 (-Tail)] contains only the lumenal domain. Soluble LAMP-1 (+/-Tail) has been reported in circulation. LAMP-1 at the cell surface has been shown to interact with E-selectin and galectin and is proposed to function in cell-cell interactions. However, the functional role(s) of soluble LAMP-1 in circulation is unclear. To investigate the functional role of soluble LAMP-1 in circulation, recombinant LAMP-1 (-Tail) and LAMP-1 (+Tail) were produced in HT1080 cells. Two immune-quantification assays were developed to distinguish between the LAMP-1 forms. The interaction and aggregation properties of the different LAMP-1 forms were investigated using the immune-quantification assays. Only LAMP-1 (+Tail) was found to aggregate and interact with plasma proteins. Plasma proteins that interact with LAMP-1 were isolated by affinity chromatography with either the recombinant LAMP-1 (-Tail) or a synthesized peptide consisting of the 14 amino acids of the LAMP-1 cytoplasmic tail. Transthyretin was found to interact with the cytoplasmic tail of LAMP-1. Transthyretin exists as a homotetramer in plasma, as such may play a role in the aggregation of LAMP-1 in circulation.
dc.identifier.citationBiochemical Journal, 2004; 382(2):481-489
dc.identifier.doi10.1042/BJ20031752
dc.identifier.issn0264-6021
dc.identifier.issn1470-8728
dc.identifier.urihttp://hdl.handle.net/2440/7012
dc.language.isoen
dc.publisherPortland Press
dc.source.urihttps://doi.org/10.1042/bj20031752
dc.subjectCell Line, Tumor
dc.subjectHumans
dc.subjectFibrosarcoma
dc.subjectCholic Acids
dc.subjectPrealbumin
dc.subjectBlood Proteins
dc.subjectProtein Isoforms
dc.subjectRecombinant Proteins
dc.subjectAntigens, CD
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectAlternative Splicing
dc.subjectLysosomal Membrane Proteins
dc.titleTransthyretin interacts with the lysosome-associated membrane protein (LAMP-1) in circulation
dc.typeJournal article
pubs.publication-statusPublished

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