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Study of effector antigens of IBR virus on humoralimmunity

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dc.contributor.authorHemmatzadeh, F.
dc.contributor.authorKeyvanfar, H.
dc.contributor.authorAhmadi, M.
dc.date.issued2007
dc.description.abstractThe objective of this study was to determine the protein pattern and antigenic structure of BHV-1. Ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by SN test. All samples were cultured on cell cultures and purified by ultracentrifugation. Purified isolates were electerophorsed using SDS-PAGE method and immunoblotted. Viral antigens were detected using anti BHV-1 bovine antiserum and molecular weight of the bands were determined. All samples showed identical band number and molecular weight except for one sample which revealed an additional 120-125 KDa band. Also immunoblotting data resulted in identified of twelve bands (150, 130, 115, 97, 77, 70, 55, 45, 40, 38, 32 and 25 KDa) suggesting involvement of these antigens in evoking humoral immune response in affected cattle.
dc.description.statementofresponsibilityHemmatzadeh, F., Keyvanfar, H., Ahmadi, M.
dc.identifier.citationJournal of Veterinary Research, 2007; 62(1):57-61
dc.identifier.issn2008-2525
dc.identifier.orcidHemmatzadeh, F. [0000-0002-4572-8869]
dc.identifier.urihttp://hdl.handle.net/2440/68743
dc.language.isoen
dc.publisherUniversity of Tehran
dc.rightsCopyright status unknown
dc.subjectvirus
dc.subjectIBR
dc.subjectSDS-PAGE
dc.subjectimmunoblotting
dc.subjectantigen.
dc.titleStudy of effector antigens of IBR virus on humoralimmunity
dc.typeJournal article
pubs.publication-statusPublished

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