Effects of irradiation and non-enzymatic glycation on the fracture resistance of bovine cortical bone

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dc.contributor.authorFletcher, L.
dc.contributor.authorCodrington, J.
dc.contributor.authorParkinson, I.
dc.contributor.conferenceAustralasian Congress on Applied Mechanics (7th : 2012 : Adelaide, S.A.)
dc.date.issued2012
dc.description.abstractBone is a complex multi-phase composite having mechanical properties that are strongly dependent on its constituent parts. Components contributing towards the overall mechanical quality of bone include the organic phase (primarily Collagen I) and the inorganic phase (Hydroxyapatite). However, the individual contribution of each of the material phases towards crack propagation and the inherent fracture toughening mechanisms that are observed in bone are not well understood. Therefore, this study aims to investigate the contribution of the organic and inorganic constituents to the fracture resistance of cortical bone. Three treatment methods were selected that independently alter either the organic or inorganic components of cortical bone. The first two treatments effect the organic phase by denaturation of the collagen and formation of natural enzymatic cross-links from exposure to irradiation, and by incubation in a Ribose solution to induce Non-Enzymatic Glycation (NEG). The third treatment alters the inorganic phase by decalcification in ethyl-diamine-tetra-acetic acid (EDTA). Following treatment, the specimens were mechanically tested to obtain the fracture resistance curve as a function of crack growth using the unloading compliance method. To supplement this technique, stages of the crack growth were sequentially labelled using fluorochrome stains to observe the crack path as well as the toughening mechanisms (e.g. ligament bridging). Overall, the results of this study show that the organic phase is responsible for the toughening and fracture resistance behaviour of cortical bone while the inorganic phase contributes to its stiffness. The results of this study also suggest that the formation of natural enzymatic cross-links and protein denaturation due to irradiation are more detrimental to the fracture resistance of cortical bone than the non-enzymatic cross-links formed by NEG.
dc.description.statementofresponsibilityLloyd Fletcher, John Codrington and Ian Parkinson
dc.identifier.citationProceedings of the 7th Australasian Congress on Applied Mechanics (ACAM 7), December 10-12, 2012, Adelaide, Australia: pp.322-330
dc.identifier.isbn9781922107619
dc.identifier.urihttp://hdl.handle.net/2440/78147
dc.language.isoen
dc.publisherEngineers Australia
dc.publisher.placeCDROM / DVDROM
dc.rightsCopyright status unknown
dc.source.urihttp://search.informit.com.au/documentSummary;dn=130750115333665;res=IELENG
dc.subjectBone fracture
dc.subjectcortical bone
dc.subjectfracture resistance
dc.subjectirradiation
dc.subjectnon-enzymatic glycation
dc.titleEffects of irradiation and non-enzymatic glycation on the fracture resistance of bovine cortical bone
dc.typeConference paper
pubs.publication-statusPublished

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