MicroRNA-resistant alleles of HOMEOBOX DOMAIN-2 modify inflorescence branching and increase grain protein content of wheat

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dc.contributor.authorDixon, L.E.
dc.contributor.authorPasquariello, M.
dc.contributor.authorBadgami, R.
dc.contributor.authorLevin, K.A.
dc.contributor.authorPoschet, G.
dc.contributor.authorNg, P.Q.
dc.contributor.authorOrford, S.
dc.contributor.authorChayut, N.
dc.contributor.authorAdamski, N.M.
dc.contributor.authorBrinton, J.
dc.contributor.authorSimmonds, J.
dc.contributor.authorSteuernagel, B.
dc.contributor.authorSearle, I.R.
dc.contributor.authorUauy, C.
dc.contributor.authorBoden, S.A.
dc.date.issued2022
dc.description.abstractPlant and inflorescence architecture determine the yield potential of crops. Breeders have harnessed natural diversity for inflorescence architecture to improve yields, and induced genetic variation could provide further gains. Wheat is a vital source of protein and calories; however, little is known about the genes that regulate the development of its inflorescence. Here, we report the identification of semidominant alleles for a class III homeodomain-leucine zipper transcription factor, HOMEOBOX DOMAIN-2 (HB-2), on wheat A and D subgenomes, which generate more flower-bearing spikelets and enhance grain protein content. These alleles increase HB-2 expression by disrupting a microRNA 165/166 complementary site with conserved roles in plants; higher HB-2 expression is associated with modified leaf and vascular development and increased amino acid supply to the inflorescence during grain development. These findings enhance our understanding of genes that control wheat inflorescence development and introduce an approach to improve the nutritional quality of grain.
dc.description.statementofresponsibilityLaura E. Dixon, Marianna Pasquariello, Roshani Badgami, Kara A. Levin, Gernot Poschet, Pei Qin Ng, Simon Orford, Noam Chayut, Nikolai M. Adamski, Jemima Brinton, James Simmonds, Burkhard Steuernagel, Iain R. Searle, Cristobal Uauy, Scott A. Boden
dc.identifier.citationScience Advances, 2022; 8(19):1-16
dc.identifier.doi10.1126/sciadv.abn5907
dc.identifier.issn2375-2548
dc.identifier.issn2375-2548
dc.identifier.orcidLevin, K.A. [0000-0002-4500-983X]
dc.identifier.orcidNg, P.Q. [0000-0003-0944-1913]
dc.identifier.orcidSearle, I.R. [0000-0003-4306-9756]
dc.identifier.orcidBoden, S.A. [0000-0001-5297-4067]
dc.identifier.urihttps://hdl.handle.net/2440/135267
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science
dc.relation.granthttp://purl.org/au-research/grants/arc/FT210100810
dc.relation.granthttp://purl.org/au-research/grants/arc/DP190101303
dc.rightsCopyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
dc.source.urihttps://doi.org/10.1126/sciadv.abn5907
dc.subjectTriticum
dc.subjectPlant Proteins
dc.subjectMicroRNAs
dc.subjectGene Expression Regulation, Plant
dc.subjectAlleles
dc.subjectGenes, Homeobox
dc.subjectInflorescence
dc.subjectEdible Grain
dc.subjectGrain Proteins
dc.titleMicroRNA-resistant alleles of HOMEOBOX DOMAIN-2 modify inflorescence branching and increase grain protein content of wheat
dc.typeJournal article
pubs.publication-statusPublished

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