Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat

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dc.contributor.authorCrismani, W.
dc.contributor.authorBaumann, U.
dc.contributor.authorSutton, T.
dc.contributor.authorShirley, N.
dc.contributor.authorWebster, T.
dc.contributor.authorSpangenberg, G.
dc.contributor.authorLangridge, P.
dc.contributor.authorAble, J.
dc.date.issued2006
dc.description.abstractBackground: Our understanding of the mechanisms that govern the cellular process of meiosis is limited in higher plants with polyploid genomes. Bread wheat is an allohexaploid that behaves as a diploid during meiosis. Chromosome pairing is restricted to homologous chromosomes despite the presence of homoeologues in the nucleus. The importance of wheat as a crop and the extensive use of wild wheat relatives in breeding programs has prompted many years of cytogenetic and genetic research to develop an understanding of the control of chromosome pairing and recombination. The rapid advance of biochemical and molecular information on meiosis in model organisms such as yeast provides new opportunities to investigate the molecular basis of chromosome pairing control in wheat. However, building the link between the model and wheat requires points of data contact. Results: We report here a large-scale transcriptomics study using the Affymetrix wheat GeneChip® aimed at providing this link between wheat and model systems and at identifying early meiotic genes. Analysis of the microarray data identified 1,350 transcripts temporally-regulated during the early stages of meiosis. Expression profiles with annotated transcript functions including chromatin condensation, synaptonemal complex formation, recombination and fertility were identified. From the 1,350 transcripts, 30 displayed at least an eight-fold expression change between and including pre-meiosis and telophase II, with more than 50% of these having no similarities to known sequences in NCBI and TIGR databases. Conclusion: This resource is now available to support research into the molecular basis of pairing and recombination control in the complex polyploid, wheat.
dc.identifier.citationBMC Genomics, 2006; 7(267):1-17
dc.identifier.doi10.1186/1471-2164-7-267
dc.identifier.issn1471-2164
dc.identifier.issn1471-2164
dc.identifier.orcidBaumann, U. [0000-0003-1281-598X]
dc.identifier.orcidShirley, N. [0000-0001-8114-9891]
dc.identifier.orcidLangridge, P. [0000-0001-9494-400X]
dc.identifier.orcidAble, J. [0000-0003-3207-6301]
dc.identifier.urihttp://hdl.handle.net/2440/34188
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.rights© 2006 Crismani et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttp://www.biomedcentral.com/1471-2164/7/267
dc.subjectTriticum
dc.subjectDNA, Plant
dc.subjectDNA, Complementary
dc.subjectRNA, Plant
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPolymerase Chain Reaction
dc.subjectChromosome Pairing
dc.subjectMeiosis
dc.subjectTranscription, Genetic
dc.subjectGene Expression Regulation, Plant
dc.subjectRecombination, Genetic
dc.subjectFertility
dc.subjectPolyploidy
dc.subjectGenes, Plant
dc.subjectGenome, Plant
dc.titleMicroarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat
dc.typeJournal article
pubs.publication-statusPublished

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