Photoswitchable calcium sensor: ‘On’–‘Off’ sensing in cells or with microstructured optical fibers

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dc.contributor.authorHeng, S.
dc.contributor.authorMak, A.
dc.contributor.authorKostecki, R.
dc.contributor.authorZhang, X.
dc.contributor.authorPei, J.
dc.contributor.authorStubing, D.
dc.contributor.authorEbendorff-Heidepriem, H.
dc.contributor.authorAbell, A.
dc.date.issued2017
dc.description.abstractCalcium is a ubiquitous intracellular signaling ion that plays a critical role in the modulation of fundamental cellular processes. A detailed study of these processes requires selective and reversible sensing of Ca2+ and an ability to quantify and monitor concentration changes in a biological setting. Three new, rationally designed, synthesized and photoswitchable spiropyran-based reversible sensors for Ca2+ are reported. Sensor 1a is highly selective for Ca2+ with an improved profile relative to the other two analogues, 1b and 1c. Formation of the merocyanine–Ca2+ complex is proportional to an increase in Ca2+ released from HEK293 cells on stimulation with ionomycin. The photophysical processes surrounding the binding of Ca2+ to compound 1a were further explored using computational methods based on density functional theory (DFT). The ability of sensor 1a to bind Ca2+ and photoswitch reversibly was also characterized using silica suspended-core microstructured optical fiber (SCF). These SCF experiments (with 100 nM Ca2+) represent a first step toward developing photoswitchable, minimally invasive and highly sensitive Ca2+ sensing platforms for use in a biological setting.
dc.description.statementofresponsibilitySabrina Heng, Adrian M. Mak, Roman Kostecki, Xiaozhou Zhang Jinxin Pei, Daniel B.Stubing, Heike Ebendorff-Heidepriem, Andrew D.Abell
dc.identifier.citationSensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 2017; 252:965-972
dc.identifier.doi10.1016/j.snb.2017.06.051
dc.identifier.issn0925-4005
dc.identifier.issn0925-4005
dc.identifier.orcidKostecki, R. [0000-0001-5822-8401]
dc.identifier.orcidPei, J. [0000-0003-2453-929X]
dc.identifier.orcidEbendorff-Heidepriem, H. [0000-0002-4877-7770]
dc.identifier.orcidAbell, A. [0000-0002-0604-2629]
dc.identifier.urihttp://hdl.handle.net/2440/111112
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100003
dc.rights© 2017 Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.snb.2017.06.051
dc.subjectSpiropyran; calcium sensing; density functional theory; suspended-core microstructured optical fiber
dc.titlePhotoswitchable calcium sensor: ‘On’–‘Off’ sensing in cells or with microstructured optical fibers
dc.typeJournal article
pubs.publication-statusPublished

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