Vitamin D upregulates the macrophage complement receptor immunoglobulin in innate immunity to microbial pathogens

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dc.contributor.authorSmall, A.G.
dc.contributor.authorHarvey, S.
dc.contributor.authorKaur, J.
dc.contributor.authorPutty, T.
dc.contributor.authorQuach, A.
dc.contributor.authorMunawara, U.
dc.contributor.authorPerveen, K.
dc.contributor.authorMcPhee, A.
dc.contributor.authorHii, C.S.
dc.contributor.authorFerrante, A.
dc.date.issued2021
dc.description.abstractVitamin D deficiency remains a global concern. This 'sunshine' vitamin is converted through a multistep process to active 1,25-dihydroxyvitamin D₃ (1,25D), the final step of which can occur in macrophages. Here we demonstrate a role for vitamin D in innate immunity. The expression of the complement receptor immunoglobulin (CRIg), which plays an important role in innate immunity, is upregulated by 1,25D in human macrophages. Monocytes cultured in 1,25D differentiated into macrophages displaying increased CRIg mRNA, protein and cell surface expression but not in classical complement receptors, CR3 and CR4. This was associated with increases in phagocytosis of complement opsonised Staphylococcus aureus and Candida albicans. Treating macrophages with 1,25D for 24 h also increases CRIg expression. While treating macrophages with 25-hydroxyvitamin D3 does not increase CRIg expression, added together with the toll like receptor 2 agonist, triacylated lipopeptide, Pam3CSK4, which promotes the conversion of 25-hydroxyvitamin D₃ to 1,25D, leads to an increase in CRIg expression and increases in CYP27B1 mRNA. These findings suggest that macrophages harbour a vitamin D-primed innate defence mechanism, involving CRIg.
dc.description.statementofresponsibilityAnnabelle G. Small, Sarah Harvey, Jaspreet Kaur, Trishni Putty, Alex Quach, Usma Munawara, Khalida Perveen, Andrew McPhee, Charles S. Hii, Antonio Ferrante
dc.identifier.citationCommunications Biology, 2021; 4(1):401-1-401-7
dc.identifier.doi10.1038/s42003-021-01943-3
dc.identifier.issn2399-3642
dc.identifier.issn2399-3642
dc.identifier.orcidSmall, A.G. [0000-0001-6275-2847]
dc.identifier.orcidQuach, A. [0000-0003-1912-7581]
dc.identifier.orcidPerveen, K. [0000-0002-1499-1736]
dc.identifier.orcidMcPhee, A. [0000-0003-3820-5696]
dc.identifier.orcidHii, C.S. [0000-0002-7107-8935]
dc.identifier.orcidFerrante, A. [0000-0002-2581-6407]
dc.identifier.urihttps://hdl.handle.net/2440/133769
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.grantNHMRC
dc.rights© Crown 2021. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s42003-021-01943-3
dc.subjectMacrophages; vitamin D
dc.subject.meshMacrophages
dc.subject.meshCalcitriol
dc.subject.meshImmunoglobulins
dc.subject.meshReceptors, Complement 3b
dc.subject.meshUp-Regulation
dc.subject.meshImmunity, Innate
dc.titleVitamin D upregulates the macrophage complement receptor immunoglobulin in innate immunity to microbial pathogens
dc.typeJournal article
pubs.publication-statusPublished

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