Characterization of human leucocytes bearing the IL-3 receptor

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dc.contributor.authorMacardle, P.
dc.contributor.authorChen, Z.
dc.contributor.authorShih, C.
dc.contributor.authorHuang, C.
dc.contributor.authorWeedon, H.
dc.contributor.authorSun, Q.
dc.contributor.authorLopez, A.
dc.contributor.authorZola, H.
dc.date.issued1996
dc.description.abstractHuman leucocytes from peripheral blood and tonsil were examined for the presence of the IL-3 receptor using monoclonal antibodies directed to epitopes of the alpha and beta chains of the receptor. We found that the beta chain, common to IL-3, IL-5, and GM-CSF, was either present at low levels or not detected on the majority of peripheral blood and tonsil B lymphocytes, while the alpha chain showed a distinct but restricted distribution. In peripheral blood the IL-3R alpha chain was limited to a subpopulation of peripheral B lymphocytes and a population of cells which lack lineage-specific markers. Dimly staining cells were identified as B lymphocytes as they coexpressed CD19, CD20, CD22, CD24, and HLA-DR. A brightly staining population lacks T and B lymphocyte, NK specific, and macrophage lineage markers but expresses CD9, CD45RO, CD26, and, in a proportion of cells, CD36 and CD60. This population remains unclassified. In tonsil tissue IL-3R alpha chain expression was strongest on B lymphocytes present in the T cell rich areas of tonsillar tissue. The IL-3R alpha bearing B tonsil cells included cells in both CD23 and IgD positive and negative populations. The phenotype of the IL-3R alpha positive B cells defines them as a population of B lymphocytes distinct from previously characterized cells in the lymphoid architecture. Lymphoblastoid cell lines with a corresponding phenotype were also identified.
dc.description.statementofresponsibilityP.J. Macardle, Z. Chen, C-Y. Shih, C-M. Huang, H. Weedon, Q. Sun, A.F. Lopez, H. Zola
dc.identifier.citationCellular Immunology, 1996; 168(1):59-68
dc.identifier.doi10.1006/cimm.1996.0049
dc.identifier.issn0008-8749
dc.identifier.issn1090-2163
dc.identifier.orcidLopez, A. [0000-0001-7430-0135]
dc.identifier.urihttp://hdl.handle.net/2440/7107
dc.language.isoen
dc.publisherACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
dc.source.urihttps://doi.org/10.1006/cimm.1996.0049
dc.subjectB-Lymphocytes
dc.subjectLeukocytes, Mononuclear
dc.subjectCell Line
dc.subjectHumans
dc.subjectReceptors, Interleukin-3
dc.subjectAntigens, CD
dc.subjectCell Separation
dc.subjectLymphocyte Activation
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectPalatine Tonsil
dc.titleCharacterization of human leucocytes bearing the IL-3 receptor
dc.typeJournal article
pubs.publication-statusPublished

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