Genes that mediate starch metabolism in developing and germinated barley grain

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dc.contributor.authorCollins, H.M.
dc.contributor.authorBetts, N.S.
dc.contributor.authorDockter, C.
dc.contributor.authorBerkowitz, O.
dc.contributor.authorBraumann, I.
dc.contributor.authorCuesta-Seijo, J.A.
dc.contributor.authorSkadhauge, B.
dc.contributor.authorWhelan, J.
dc.contributor.authorBulone, V.
dc.contributor.authorFincher, G.B.
dc.date.issued2021
dc.descriptionPublished: 01 March 2021
dc.description.abstractStarch is synthesized in the endosperm of developing barley grain, where it functions as the primary source of stored carbohydrate. In germinated grain these starch reserves are hydrolyzed to small oligosaccharides and glucose, which are transported to the embryo to support the growth of the developing seedling. Some of the mobilized glucose is transiently stored as starch in the scutellum of germinated grain. These processes are crucial for early seedling vigor, which is a key determinant of crop productivity and global food security. Several starch synthases (SS), starch-branching enzymes (SBEs), and starch debranching enzymes (isoamylases, ISA), together with a limit dextrinase (LD), have been implicated in starch synthesis from nucleotide-sugar precursors. Starch synthesis occurs both in the developing endosperm and in the scutellum of germinated grain. For the complete depolymerization of starch to glucose, α-amylase (Amy), β-amylase (Bmy), isoamylase (ISA), limit dextrinase (LD), and α-glucosidase (AGL) are required. Most of these enzymes are encoded by gene families of up to 10 or more members. Here RNA-seq transcription data from isolated tissues of intact developing and germinated barley grain have allowed us to identify the most important, specific gene family members for each of these processes in vivo and, at the same time, we have defined in detail the spatio-temporal coordination of gene expression in different tissues of the grain. A transcript dataset for 81,280 genes is publicly available as a resource for investigations into other cellular and biochemical processes that occur in the developing grain from 6 days after pollination.
dc.description.statementofresponsibilityHelen M. Collins, Natalie S. Betts, Christoph Dockter, Oliver Berkowitz, Ilka Braumann, Jose A. Cuesta-Seijo, Birgitte Skadhauge, James Whelan, Vincent Bulone and Geoffrey B. Fincher
dc.identifier.citationFrontiers in Plant Science, 2021; 12:641325-1-641325-15
dc.identifier.doi10.3389/fpls.2021.641325
dc.identifier.issn1664-462X
dc.identifier.issn1664-462X
dc.identifier.orcidCollins, H.M. [0000-0003-3885-7707]
dc.identifier.orcidBetts, N.S. [0000-0002-3345-8160]
dc.identifier.orcidBulone, V. [0000-0002-9742-4701] [0000-0003-2809-4160]
dc.identifier.urihttp://hdl.handle.net/2440/129951
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.granthttp://purl.org/au-research/grants/arc/LP160100700
dc.relation.granthttp://purl.org/au-research/grants/arc/CE1101007
dc.rightsCopyright © 2021 Collins, Betts, Dockter, Berkowitz, Braumann, Cuesta-Seijo, Skadhauge, Whelan, Bulone and Fincher. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.source.urihttps://doi.org/10.3389/fpls.2021.641325
dc.subjectaleurone; endosperm; gene families; Hordeum vulgare; RNA-seq; scutellum; starch synthesis; starch degradation
dc.titleGenes that mediate starch metabolism in developing and germinated barley grain
dc.typeJournal article
pubs.publication-statusPublished

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