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Interaction of endothelial cells with plasma-polymer modified surfaces

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dc.contributor.authorWang, L.
dc.contributor.authorLi, J.
dc.contributor.authorCoad, B.R.
dc.contributor.authorMcFarland, C.D.
dc.contributor.authorNordon, R.E.
dc.date.issued2020
dc.descriptionData source: Supplementary materials, https://doi.org/10.1016/j.mtla.2020.100613
dc.description.abstractEndothelialisation of implantable vascular grafts and stents is directed by the adsorbed protein layer. Chemical and mechanical cues sensed at the biomaterial surface by endothelial cells determine their attachment, survival and proliferation. Given the multiplicity of possible interactions we describe the novel application of live cell imaging, factorial analysis and single molecule imaging to investigate higher order interactions between surface chemistry and adsorbed proteins influencing endothelial cell (EC) adhesion dynamics. EC fates were tracked by time-lapse imaging of cell contact area on plasma polymer modified surfaces. The combinatorial effect of plasma polymer chemistry and adsorbed proteins was characterised by factorial experimental design and analysis. Single molecule imaging and counting was used for the first time to quantify binding of fluorescently labelled albumin to plasma polymerized surfaces: Modified glass surfaces with thin plasma polymer coatings rich in primary amine groups had a high affinity for albumin (~2, 650 molecules/μm2) while plasma polymer surfaces functionalised with hydroxyl groups had very low levels of albumin binding (22–30 molecules/μm2). Plasma polymer coatings rich in primary amine groups also promoted endothelial cell adhesion and was superior to tissue culture plastic. An interaction between albumin, heparin and fibronectin promoted adhesion to amine plasma polymer coated glass, while hydroxyl plasma polymer coated glass prevented EC attachment. Tracking cell-specific interactions with the adsorbed protein layer by time-lapse microscopy is more predictive of in vivo cellular responses to biomaterials compared to studies that only measure protein adsorption
dc.identifier.citationMaterialia, 2020; 9(100613)
dc.identifier.doi10.1016/j.mtla.2020.100613
dc.identifier.issn2589-1529
dc.identifier.issn2589-1529
dc.identifier.orcidCoad, B.R. [0000-0003-3297-3977]
dc.identifier.urihttps://hdl.handle.net/11541.2/141233
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/631931
dc.rightsCopyright 2020 Crown Copyright, published by Elsevier
dc.source.urihttps://doi.org/10.1016/j.mtla.2020.100613
dc.subjectplasma polymer surface modification
dc.subjectsingle molecule imaging
dc.subjectendothelial cells
dc.subjectlive cell imaging
dc.titleInteraction of endothelial cells with plasma-polymer modified surfaces
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916375376201831

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