Granulocyte-macrophage colony-stimulating factor enhances EBV-induced synthesis of chemotactic factors in human neutrophils.

Simple item page
dc.contributor.authorRoberge, C.
dc.contributor.authorMcColl, S.
dc.contributor.authorLarochelle, B.
dc.contributor.authorGosselin, J.
dc.date.issued1998
dc.description.abstractWe have recently demonstrated that EBV binds to human neutrophils and stimulates a wide range of activities, including homeotypic aggregation, total RNA synthesis, and expression of the chemokines IL-8 and macrophage inflammatory protein-1alpha (MIP-1alpha). Neutrophil function is also known to be modulated by priming with granulocyte-macrophage colony-stimulating factor (GM-CSF). We have therefore investigated the modulation of EBV-induced activation of human neutrophils by GM-CSF. Treatment of neutrophils with GM-CSF before EBV activation enhanced the production of both MIP-1alpha and IL-8. The IL-8 produced under these conditions was biologically active as determined in the calcium mobilization assay. GM-CSF was also found to increase the ability of EBV to prime neutrophils for increased leukotriene B4 (LTB4) synthesis. Prior treatment of GM-CSF with neutralizing Abs inhibited these effects. GM-CSF also increased the specific binding of FITC-EBV to the neutrophil surface, as evaluated by fluorocytometry. Local production of GM-CSF in tissues invaded by EBV could therefore serve to potentiate a host defense mechanism directed toward the destruction of the infectious virus via increased production of chemotactic factors. Since both IL-8 and MIP-1alpha are reported to be chemoattractants in vitro for T cells and T and B cells, respectively, the ability of EBV to induce their production by neutrophils may enhance its ability to infect B and T lymphocytes via increased recruitment to sites of infection.
dc.identifier.citationJournal of Immunology, 1998; 160(5):2442-2448
dc.identifier.doi10.4049/jimmunol.160.5.2442
dc.identifier.issn0022-1767
dc.identifier.issn1550-6606
dc.identifier.orcidMcColl, S. [0000-0003-0949-4660]
dc.identifier.urihttp://hdl.handle.net/2440/11627
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.source.urihttps://doi.org/10.4049/jimmunol.160.5.2442
dc.subjectNeutrophils
dc.subjectHumans
dc.subjectHerpesvirus 4, Human
dc.subjectCalcium
dc.subjectLeukotriene B4
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor
dc.subjectRNA, Messenger
dc.subjectInterleukin-8
dc.subjectMacrophage Inflammatory Proteins
dc.subjectChemotactic Factors
dc.subjectAdjuvants, Immunologic
dc.subjectNeutrophil Activation
dc.subjectChemokine CCL3
dc.subjectChemokine CCL4
dc.titleGranulocyte-macrophage colony-stimulating factor enhances EBV-induced synthesis of chemotactic factors in human neutrophils.
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Microbiology and Immunology publications