Development and characterization of primary cultures of smooth muscle cells from the fibromuscular stroma of the guinea pig prostate
Date
1989
Authors
Ricciardelli, C.
Horsfall, D.J.
Skinner, J.M.
Henderson, D.W.
Marshall, V.R.
Tilley, W.D.
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Journal article
Citation
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1989; 25(11):1016-1024
Statement of Responsibility
Carmela Ricciardelli, David J. Horsfall, John M. Skinner, Douglas W. Henderson, Villis R. Marshall, Wayne D. Tilley
Conference Name
Abstract
Primary cultures of smooth muscle cells (SMCs) were obtained by a two-step enzymatic digestion of guinea pig prostatic stroma. Ultrastructural morphology and growth characteristics of these cells conformed to those reported for SMCs isolated from vascular and visceral tissue sources. Electron microscopic examination indicated that the cells assumed modified myofibroblastoid features in culture. Microfilaments with associated dense bodies were markedly depleted in cultured smooth muscle cells, in comparison with those of the parent tissue. Cultured cells also possessed increased content of rough endoplasmic reticulum indicating the increased secretory or protein-synthetic capacity of the cells. Immunoperoxidase staining for cytoskeletal markers using monoclonal antibodies to desmin and vimentin supported the ultrastructural observations, suggesting a decline in desmin-staining intermediate filaments during "modulation" to the myofibroblastoid form. Despite this depletion of smooth muscle-specific differentiation markers and reversion to more general mesenchymal properties, the cells retained the ability to contract on challenge with norepinephrine, and grew in the characteristic "hill and valley" pattern on attaining confluence. Inasmuch as the estrogen and androgen receptor expression of the parent stromal tissue is also retained, these primary cell cultures should provide a useful model to study regulation of prostatic development.
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© Tissue Culture Association, Inc 1989.