Sperm DNA damage is associated with assisted reproductive technology pregnancy
Date
2008
Authors
Bakos, H.
Thompson, J.
Inglis, D.
Lane, M.
Editors
Advisors
Journal Title
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Volume Title
Type:
Journal article
Citation
International Journal of Andrology, 2008; 31(5):518-526
Statement of Responsibility
Hassan W. Bakos, Jeremy G Thompson, Deanne Feil, Michelle Lane
Conference Name
Abstract
The literature suggests an association between sperm DNA damage and assisted reproductive technology (ART) outcomes. However, previous studies involved the transfer of multiple embryos, which has complicated the interpretation of the results. The aim of this study was to determine the relationship between the levels of sperm DNA damage and fertilization rate, embryo development as well as pregnancy outcome, following single embryo transfer. Patients (n = 113) undergoing in vitro fertilization (IVF) (n = 45) and intra-cytoplasmic sperm injection (ICSI) (n = 68) were assessed for their levels of sperm DNA damage in the sample used for insemination. DNA damage was determined using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling (TUNEL). The relationship between DNA damage and outcomes were assessed using regression analysis. Overall data showed no association between sperm DNA damage and fertilization rate, or embryo development in vitro. However, when IVF was the insemination method, there was a significant negative correlation between fertilization rates and sperm DNA damage (p < 0.05). When ICSI was the insemination technique, low sperm DNA damage was associated with successful pregnancy (37.8 ± 5.7% DNA damaged sperm) compared with failed implantation (52.9 ± 3.9% DNA damaged sperm, p < 0.05). Our results suggest that sperm DNA damage as measured by the TUNEL assay may provide an indicator for patients with poor fertilization rates and/or those unable to achieve pregnancy following ART treatment.
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Dissertation Note
Provenance
Published Online: 5 Sep 2007
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© 2007 The Authors. Journal compilation © 2007 European Academy of Andrology