Microbial cell lysis and nucleic acid extraction via nanofluidic channel

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dc.contributor.authorKant, K.
dc.contributor.authorYoo, J.
dc.contributor.authorAmos, S.
dc.contributor.authorErkelens, M.
dc.contributor.authorPriest, C.
dc.contributor.authorShapter, J.
dc.contributor.authorLosic, D.
dc.date.issued2015
dc.description.abstractThis paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.
dc.description.statementofresponsibilityKrishna Kant, Jeongha Yoo, Steven Amos, Mason Erkelens, Craig Priest, Joe G. Shapter and Dusan Losic
dc.identifier.citationRSC Advances: an international journal to further the chemical sciences, 2015; 5(30):23886-23891
dc.identifier.doi10.1039/c5ra01336d
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069
dc.identifier.orcidLosic, D. [0000-0002-1930-072X]
dc.identifier.urihttp://hdl.handle.net/2440/91317
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.granthttp://purl.org/au-research/grants/arc/FT110100711
dc.source.urihttps://doi.org/10.1039/c5ra01336d
dc.titleMicrobial cell lysis and nucleic acid extraction via nanofluidic channel
dc.typeJournal article
pubs.publication-statusPublished

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