Discovery of a potent, highly selective, and orally bioavailable inhibitor of CDK8 through a structure-based optimisation

Date

2021

Authors

Yu, M.
Long, Y.
Yang, Y.
Li, M.
Teo, T.
Noll, B.
Philip, S.
Wang, S.

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Journal article

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European Journal of Medicinal Chemistry, 2021; 218(113391)

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Abstract

CDK8 is deregulated in multiple types of human cancer and is viewed as a therapeutic target for the treatment of the disease. Accordingly, the search for small-molecule inhibitors of CDK8 is being intensified. Capitalising on our initial discovery of AU1-100, a potent CDK8 inhibitor yet with a limited degree of kinase selectivity, a structure-based optimisation was carried out, with a series of new multi-substituted pyridines rationally designed, chemically prepared and biologically evaluated. Such endeavour has culminated in the identification of 42, a more potent CDK8 inhibitor with superior kinomic selectivity and oral bioavailability. The mechanism underlying the anti-proliferative effect of 42 on MV4-11 cells was studied, revealing that the compound arrested the G1 cell cycle and triggered apoptosis. The low risk of hepato- and cardio-toxicity of 42 was estimated. These findings merit further investigation of 42 as a targeted cancer therapeutic.

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Data source: Supplementary data, https://doi.org/10.1016/j.ejmech.2021.113391

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Copyright 2021 Elsevier Masson Access Condition Notes: Accepted manuscript available 1 April 2023

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