The influence of intracellular polyglucose and prior growth rate on the survival of Fusobacterium nucleatum under starvation conditions
| dc.contributor.author | Rogers, A. | |
| dc.contributor.author | Zilm, P. | |
| dc.date.issued | 1995 | |
| dc.description.abstract | Grown in a chemically defined medium containing glucose at a dilution rate of D = 0.065 h-1, Fusobacterium nucleatum D212B-2 produced large amounts of intracellular polyglucose. Aliquots of this culture were starved by anaerobic incubation at 37 degrees C and at various times, assayed for intracellular polyglucose content and viability. This protocol was repeated using cells grown under the same conditions in a chemically defined medium, a medium lacking carbohydrate and in which the organism produced no intracellular polyglucose. Both cultures had 50% survival time values of about 1.5 h and were not eliminated even after 32 h of starvation. It was, therefore concluded that starvation-survival is not influenced by intracellular polyglucose. Starvation-survival was also determined for cells grown in a chemically defined medium at D = 0.048 h-1 and D = 0.12 h-1. The faster-grown cells had a 50% survival time of 3.8 h but were completely eliminated after 8-16 h of starvation. In contrast, slower-grown cells had a 50% survival time of 1.5 h but were not completely eliminated after 32 h of starvation. This illustrates the importance of cell history and technique standardization in comparing the starvation-survival of different organisms. | |
| dc.identifier.citation | Molecular Oral Microbiology, 1995; 10(2):119-121 | |
| dc.identifier.doi | 10.1111/j.1399-302X.1995.tb00130.x | |
| dc.identifier.issn | 0902-0055 | |
| dc.identifier.issn | 1399-302X | |
| dc.identifier.orcid | Zilm, P. [0000-0001-7554-9717] | |
| dc.identifier.uri | http://hdl.handle.net/2440/1614 | |
| dc.language.iso | en | |
| dc.publisher | Munksgaard | |
| dc.source.uri | https://doi.org/10.1111/j.1399-302x.1995.tb00130.x | |
| dc.subject | Fusobacterium nucleatum | |
| dc.subject | Glucans | |
| dc.subject | Culture Media | |
| dc.subject | Bacteriological Techniques | |
| dc.subject | Adaptation, Physiological | |
| dc.subject | Cell Division | |
| dc.subject | Anaerobiosis | |
| dc.title | The influence of intracellular polyglucose and prior growth rate on the survival of Fusobacterium nucleatum under starvation conditions | |
| dc.type | Journal article | |
| pubs.publication-status | Published |