Emu oil expedites small intestinal repair following 5-fluorouracil-induced mucositis in rats

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dc.contributor.authorMashtoub, S.
dc.contributor.authorTran, C.
dc.contributor.authorHowarth, G.
dc.date.issued2013
dc.description.abstractMucositis resulting from cancer chemotherapy is characterized by intestinal inflammation and ulceration. Previously, emu oil (EO) improved intestinal architecture (Br J Nutr, 2010) in a rat model of chemotherapy-induced mucositis. We investigated EO for its further potential to promote intestinal repair in this mucositis model. Female Dark Agouti rats (n = 8/group) were gavaged with water, olive oil (OO) or EO once daily (1 mL), injected with 5-fluorouracil (5-FU) or saline on day 5 and euthanized on day 8, 9, 10 or 11. Intestinal villus height (VH) and crypt depth (CD), neutral mucin-secreting goblet cell (GC) count, myeloperoxidase (MPO) activity and selected cytokines were quantified; P < 0.05 was considered significant. In 5-FU-injected rats, only EO administration significantly increased VH in the ileum (day 8), jejunum and jejunum–ileum junction (days 8 and 9) compared to 5-FU controls (P < 0.05). GC count was significantly reduced by 5-FU (jejunum: days 8 and 9; ileum: day 8; P < 0.05) and EO increased ileal GC on days 10 and 11 compared to 5-FU controls. MPO activity was significantly increased in jejunum (days 8 and 9) and ileum (day 8) following 5-FU injection, compared to normal controls (P < 0.05). Both EO and OO significantly reduced jejunal MPO on days 8 and 9; however, only EO decreased ileal MPO on day 8. Cytokine levels were not significantly affected by either oil or 5-FU administration at the day 8 time point. Promotion of repair from injury could represent a new mechanism of action for EO, suggesting potential as an adjunct to conventional treatment approaches for cancer management.
dc.description.statementofresponsibilitySuzanne Mashtoub, Cuong D Tran and Gordon S Howarth
dc.identifier.citationExperimental Biology and Medicine, 2013; 238(11):1305-1317
dc.identifier.doi10.1177/1535370213493718
dc.identifier.issn1535-3702
dc.identifier.issn1535-3699
dc.identifier.orcidMashtoub, S. [0000-0001-7308-8371]
dc.identifier.orcidTran, C. [0000-0001-9220-2715]
dc.identifier.orcidHowarth, G. [0000-0001-6979-6084]
dc.identifier.urihttp://hdl.handle.net/2440/81690
dc.language.isoen
dc.publisherBlackwell Science Inc
dc.rights© 2013 by the Society for Experimental Biology and Medicine
dc.source.urihttps://doi.org/10.1177/1535370213493718
dc.subjectChemotherapy
dc.subjectintestinal mucositis
dc.subjectemu oil
dc.subjectrepair
dc.subjectrat
dc.titleEmu oil expedites small intestinal repair following 5-fluorouracil-induced mucositis in rats
dc.typeJournal article
pubs.publication-statusPublished

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