Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions

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dc.contributor.authorHackenberg, M.
dc.contributor.authorShi, B.
dc.contributor.authorGustafson, P.
dc.contributor.authorLangridge, P.
dc.date.issued2013
dc.description17 p.
dc.description.abstractBACKGROUND: miR399 and miR827 are both involved in conserved phosphorus (P) deficiency signalling pathways. miR399 targets the PHO2 gene encoding E2 enzyme that negatively regulates phosphate uptake and root-to-shoot allocation, while miR827 targets SPX-domain-containing genes that negatively regulate other P-responsive genes. However, the response of miR399 and miR827 to P conditions in barley has not been investigated. RESULTS: In this study, we investigated the expression profiles of miR399 and miR827 in barley (Hordeum vulagre L.) under P-deficient and P-sufficient conditions. We identified 10 members of the miR399 family and one miR827 gene in barley, all of which were significantly up-regulated under deficient P. In addition, we found many isomirs of the miR399 family and miR827, most of which were also significantly up-regulated under deficient P. Several isomirs of miR399 members were found to be able to cleave their predicted targets in vivo. Surprisingly, a few small RNAs (sRNAs) derived from the single-stranded loops of the hairpin structures of MIR399b and MIR399e-1 were also found to be able to cleave their predicted targets in vivo. Many antisense sRNAs of miR399 and a few for miR827 were also detected, but they did not seem to be regulated by P. Intriguingly, the lowest expressed member, hvu-miR399k, had four-fold more antisense sRNAs than sense sRNAs, and furthermore under P sufficiency, the antisense sRNAs are more frequent than the sense sRNAs. We identified a potential regulatory network among miR399, its target HvPHO2 and target mimics HvIPS1 and HvIPS2 in barley under P-deficient and P-sufficient conditions. CONCLUSIONS: Our data provide an important insight into the mechanistic regulation and function of miR399, miR827 and their isomirs in barley under different P conditions.
dc.description.statementofresponsibilityMichael Hackenberg, Bu-Jun Shi, Perry Gustafson and Peter Langridge
dc.identifier.citationBMC Plant Biology, 2013; 13(214):1-17
dc.identifier.doi10.1186/1471-2229-13-214
dc.identifier.issn1471-2229
dc.identifier.issn1471-2229
dc.identifier.orcidLangridge, P. [0000-0001-9494-400X]
dc.identifier.urihttp://hdl.handle.net/2440/82134
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.relation.grantARC
dc.rights© 2013 Hackenberg et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1186/1471-2229-13-214
dc.subjectBarley
dc.subjectphosphorus
dc.subjectmir399
dc.subjectmir827
dc.subjectisomir
dc.subjectantisense
dc.subjecttargets
dc.subjectdifferential expression
dc.titleCharacterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions
dc.typeJournal article
pubs.publication-statusPublished

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