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Temporal changes in TFF3 expression and jejunal morphology during methotrexate-induced damage and repair

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dc.contributor.authorXian, C.
dc.contributor.authorHowarth, G.
dc.contributor.authorMardell, C.
dc.contributor.authorCool, J.
dc.contributor.authorFamilari, M.
dc.contributor.authorRead, L.
dc.contributor.authorGiraud, A.
dc.date.issued1999
dc.description.abstractTrefoil factor TFF3 has been implicated in intestinal protection and repair. This study investigated the spatiotemporal relationship between TFF3 expression and morphological changes during intestinal damage and repair in a rat model of methotrexate-induced small intestinal mucositis. Intestinal tissues from rats with mucositis were collected daily for 10 days. Mucosal damage was characterized by an initial decrease in cell proliferation resulting in crypt loss, villus atrophy, and depletion of goblet cells, followed by hyperproliferation that lead to crypt and villus regeneration and mucous cell repopulation. TFF3 mRNA levels increased marginally during histological damage, and the cell population expressing TFF3 mRNA expanded from the usual goblet cells to include some nongoblet epithelial cells before goblet cell repopulation. TFF3 peptide, however, was depleted during histological damage and normalized during repair, mirroring the disappearance and repopulation of goblet cells. Although there is no temporal relationship between TFF3 levels and crypt hyperproliferation, confirming the nonmitogenic nature of TFF3, the coincidental normalization of TFF3 peptide with repopulation of goblet cells and mucin production after proliferative overshoot suggests that TFF3 may play a role in the remodeling phase of repair.
dc.identifier.citationAmerican Journal of Physiology - Gastrointestinal and Liver Physiology, 1999; 277(4 40-4):G785-G795
dc.identifier.doi10.1152/ajpgi.1999.277.4.g785
dc.identifier.issn0193-1857
dc.identifier.issn1522-1547
dc.identifier.orcidXian, C. [0000-0002-8467-2845]
dc.identifier.orcidHowarth, G. [0000-0001-6979-6084]
dc.identifier.urihttp://hdl.handle.net/2440/6948
dc.language.isoen
dc.publisherAMER PHYSIOLOGICAL SOC
dc.source.urihttps://doi.org/10.1152/ajpgi.1999.277.4.g785
dc.subjectGoblet Cells
dc.subjectJejunum
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectMethotrexate
dc.subjectPeptides
dc.subjectProteins
dc.subjectMuscle Proteins
dc.subjectMucins
dc.subjectRNA, Messenger
dc.subjectNucleic Acid Synthesis Inhibitors
dc.subjectWound Healing
dc.subjectCell Division
dc.subjectUp-Regulation
dc.subjectTime Factors
dc.subjectMale
dc.subjectTrefoil Factor-3
dc.titleTemporal changes in TFF3 expression and jejunal morphology during methotrexate-induced damage and repair
dc.typeJournal article
pubs.publication-statusPublished

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