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Telomere dynamics during replicative senescence are not directly modulated by conditions of oxidative stress in IMR90 fibroblast cells

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dc.contributor.authorBritt Compton, B.
dc.contributor.authorWyllie, F.
dc.contributor.authorRowson, J.
dc.contributor.authorCapper, R.
dc.contributor.authorJones, R.E.
dc.contributor.authorBaird, D.M.
dc.date.issued2009
dc.description.abstractThe replicative lifespan of many cell types is determined by the length of telomeres in the initiating cell population. In 20% oxygen, IMR90 cells have a shorter replicative lifespan compared to that achieved in conditions that lower the levels of oxidative stress. We sought to address the role of telomere dynamics in determining the replicative lifespan of IMR90 cells. We analysed clonal populations cultured in parallel in 3 and 20% oxygen. We observed that, at senescence, telomere length was shorter in 3% oxygen and this was proportional to the lifespan extension. We observed no detectable difference in the rate of telomere erosion in the two culture conditions, however as the cells approached senescence the growth rate of the cultures slowed with a commensurate increase in the rate of telomere erosion. We conclude that, in 20% oxygen senescence of IMR90 is telomere-independent, but telomere-dependent in 3% oxygen.
dc.identifier.citationBiogerontology, 2009; 10(6):683-693
dc.identifier.doi10.1007/s10522-009-9216-4
dc.identifier.issn1389-5729
dc.identifier.issn1573-6768
dc.identifier.urihttps://hdl.handle.net/1959.8/162531
dc.language.isoen
dc.publisherSpringer Netherlands
dc.relation.fundingCancer Research UK
dc.rightsCopyright 2009 Springer Science+Business Media BV
dc.source.urihttps://doi.org/10.1007/s10522-009-9216-4
dc.subjectoxidative stress
dc.subjectreactive oxygen species
dc.subjectreplicative lifespan
dc.subjectsenescence
dc.subjecttelomerase
dc.subjecttelomere
dc.titleTelomere dynamics during replicative senescence are not directly modulated by conditions of oxidative stress in IMR90 fibroblast cells
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915910755001831

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