Current studentsExisting staffContact us
 

Effects of simulated bleeding in an in vitro nasal fibroblast wound healing model

Simple item page
dc.contributor.authorBeule, A.
dc.contributor.authorAthanasiadis, T.
dc.contributor.authorField, J.
dc.contributor.authorHosemann, W.
dc.contributor.authorWormald, P.
dc.contributor.authorTan, L.
dc.date.issued2010
dc.description.abstractBackground: We investigated the effect of simulated bleeding on plasminogen activity, matrix metalloproteinase (MMP) expression, and wound healing using a human fibroblast model. Methods: Nasal fibroblasts from three chronic rhinosinusitis (CRS) patients with nasal polyps and three controls were grown in culture and a standardized injury was created using a punch. To mimic bleeding, fibroblasts were stimulated with plasminogen (100 μg/mL), plasminogen + tranexamic acid (TA; 100 μg/mL) or media only. At 24, 48, and 72 hours after injury, we measured urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) activities and inactive and active MMP-2 and -9 expression. Results: Injury stimulated the nasal fibroblasts to express uPA and tPA and active and inactive MMP-2 and -9. In CRS patients, plasminogen significantly decreased MMP-9 expression after 48 hours (p < 0.04). In untreated fibroblasts, we observed a decrease in active MMP-9 expression, whereas plasminogen increased active MMP-9 expression after 48 hours (p < 0.04). At 24 hours, active MMP-9 expression was reduced by plasminogen ± TA (p < 0.02). Plasminogen also stimulated uPA expression in CRS patient fibroblasts after 48 hours (p < 0.04). Fibroblast proliferation occurred when exposed to plasminogen and was strongly modulated by uPA and inactive and active MMP-2. The quality of wound healing was affected by inactive MMP-2, uPA and tPA, simulation, and inhibition of bleeding. Conclusion: Activation of the plasminogen pathway and inactive MMP-2 expression tended to increase both proliferation of nasal fibroblasts and MMP-9 expression as a marker for deterioration of the quality of wound healing.
dc.description.statementofresponsibilityBeule Achim Georg, Athanasiadis Theodore, Field John, Hosemann Werner, Wormald Peter-John and Tan Lor Wai
dc.identifier.citationAmerican Journal of Rhinology and Allergy, 2010; 24(3):186-191
dc.identifier.doi10.2500/ajra.2010.24.3452
dc.identifier.issn1945-8924
dc.identifier.issn1945-8932
dc.identifier.orcidWormald, P. [0000-0001-7753-7277]
dc.identifier.urihttp://hdl.handle.net/2440/60647
dc.language.isoen
dc.publisherOcean Side Publications Inc
dc.rightsWebsite © 2010 Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.
dc.source.urihttps://doi.org/10.2500/ajra.2010.24.3452
dc.subjectBleeding
dc.subjectchronic rhinosinusitis
dc.subjectendoscopic sinus surgery
dc.subjectfibroblasts
dc.subjectmatrix metalloproteinases
dc.subjectplasminogen
dc.subjecttranexamic acid
dc.subjectwound healing model
dc.titleEffects of simulated bleeding in an in vitro nasal fibroblast wound healing model
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Surgery publications