2D and 3D mapping of microindentations in hydrated and dehydrated cortical bones using confocal laser scanning microscopy

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dc.contributor.authorYin, L.
dc.contributor.authorVenkatesan, S.
dc.contributor.authorWebb, D.
dc.contributor.authorKalyanasundaram, S.
dc.contributor.authorQin, Q.
dc.date.issued2012
dc.description.abstractWe report on responses of hydrated and dehydrated cortical bone tissues to mechanical loading applied by a Vickers indenter. The Vickers indentations were imaged in two- and three-dimensions (2D and 3D) using confocal laser scanning microscopy (CLSM) to understand mechanical behavior of bone tissues. Serial optical sections of indentation patterns of dry and wet bones were collected using CLSM. The indention surface structures were mapped using topographical CLSM imaging. The observation of CLSM shows the fundamental indentation responses for both the hydrated and dehydrated bone tissues were plastic deformation. No visible fracture was observed in the Vickers indentation patterns in the wet bone tissue, while non-propagating lamellar microcracks occurred in the dry bone tissue. This indicates that drying resulted in increased brittleness of the bone tissue. The Vickers hardness values of dry bone tissue were significantly higher than those of wet bone tissue at any applied loads (analysis of variation, ANOVA, p < 0.05). The resolution limits of confocal microscopy were also discussed for bone tissue scanning.
dc.description.statementofresponsibilityLing Yin, Sudharshan Venkatesan, Daryl Webb, Shankar Kalyanasundaram, Qing-Hua Qin
dc.identifier.citationJournal of Materials Science, 2012; 47(10):4432-4438
dc.identifier.doi10.1007/s10853-012-6301-x
dc.identifier.issn0022-2461
dc.identifier.issn1573-4803
dc.identifier.urihttp://hdl.handle.net/2440/113781
dc.language.isoen
dc.publisherSpringer
dc.rights© Springer Science+Business Media, LLC 2012
dc.source.urihttps://doi.org/10.1007/s10853-012-6301-x
dc.subjectBone tissue; cortical bone; confocal laser scanning microscopy; bone sample; radial crack
dc.title2D and 3D mapping of microindentations in hydrated and dehydrated cortical bones using confocal laser scanning microscopy
dc.typeJournal article
pubs.publication-statusPublished

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