Regulation of neutrophil-mediated killing of Staphylococcus aureus and chemotaxis by c-jun NH2 terminal kinase

Simple item page
dc.contributor.authorYeh, M.
dc.contributor.authorMukaro, V.
dc.contributor.authorHii, C.
dc.contributor.authorFerrante, A.
dc.date.issued2010
dc.description.abstractThe role of JNK in neutrophil chemotaxis and killing of microbial pathogens remains unclear. Using a recently described cell-permeable peptide inhibitor of the JNK pathway, based on the JBD of JIP-1, coupled to the protein transduction domain of HIV-TAT (TAT-JIP), in association with control peptides, we demonstrate that the JNK pathway plays a major role in regulating human neutrophil chemotaxis and killing of microbial pathogens. Serum-opsonized Staphylococcus aureus elicited JNK activation and c-jun phosphorylation. The activation of the JNK pathway and bactericidal activity were inhibited by the TAT-JIP peptide. The stimulation of oxygen radical generation by S. aureus was dependent on the JNK signaling pathway, as was the phagocytosis of serum-opsonized bacteria. Chemotaxis to activated serum complement but not random migration was inhibited by the TAT-JIP peptide. The findings demonstrate a major role for the JNK signaling pathway in neutrophil-mediated defense against microbial pathogens.
dc.description.statementofresponsibilityMei-Chun Yeh, Violet Mukaro, Charles S. Hii and Antonio Ferrante
dc.identifier.citationJournal of Leukocyte Biology, 2010; 87(5):925-932
dc.identifier.doi10.1189/jlb.0609399
dc.identifier.issn1938-3673
dc.identifier.issn1938-3673
dc.identifier.orcidHii, C. [0000-0002-7107-8935]
dc.identifier.orcidFerrante, A. [0000-0002-2581-6407]
dc.identifier.urihttp://hdl.handle.net/2440/62411
dc.language.isoen
dc.publisherFederation of American Societies for Experimental Biology
dc.rights© Society for Leukocyte Biology
dc.source.urihttps://doi.org/10.1189/jlb.0609399
dc.subjectNeutrophils
dc.subjectHL-60 Cells
dc.subjectHumans
dc.subjectStaphylococcus aureus
dc.subjectStaphylococcal Infections
dc.subjectJNK Mitogen-Activated Protein Kinases
dc.subjectEnzyme Inhibitors
dc.subjectBlotting, Western
dc.subjectImmunoprecipitation
dc.subjectChemotaxis, Leukocyte
dc.subjectPhagocytosis
dc.titleRegulation of neutrophil-mediated killing of Staphylococcus aureus and chemotaxis by c-jun NH2 terminal kinase
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Paediatrics publications