Visualization of labile zinc and its role in apoptosis of primary airway epithelial cells and cell lines

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dc.contributor.authorTruongTran, A.
dc.contributor.authorRuffin, R.
dc.contributor.authorZalewski, P.
dc.date.issued2000
dc.description.abstractThe respiratory epithelium is vulnerable to noxious substances, resulting in the shedding of cells and decreased protection. Zinc (Zn), an antioxidant and cytoprotectant, can suppress apoptosis in a variety of cells. Here we used the novel Zn-specific fluorophore Zinquin to visualize and quantify labile intracellular Zn in respiratory epithelial cells. Zinquin fluorescence in isolated ciliated tracheobronchial epithelial cells and intact epithelium from sheep and pigs revealed an intense fluorescence in the apical and mitochondria-rich cytoplasm below the cilia. Zinquin fluorescence was quenched by the Zn chelator N,N,N', N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) and increased by the Zn ionophore pyrithione. We also assessed whether changes in intracellular labile Zn would influence susceptibility of these cells to apoptosis by hydrogen peroxide. Our results confirm that Zn deficiency enhanced hydrogen peroxide-induced caspase activation from 1.24 +/- 0.12 to 2.58 +/- 0.53 units. microg protein(-1). h(-1) (P </= 0.05); Zn supplementation suppressed these effects. These findings are consistent with the hypothesis that Zn protects upper respiratory epithelial cells and may have implications for human asthma where there is hypozincemia and epithelial damage.
dc.identifier.citationAmerican Journal of Physiology - Lung Cellular and Molecular Physiology, 2000; 279(6 23-6):L1172-L1183
dc.identifier.doi10.1152/ajplung.2000.279.6.l1172
dc.identifier.issn1040-0605
dc.identifier.issn1522-1504
dc.identifier.orcidZalewski, P. [0000-0001-5196-2611]
dc.identifier.urihttp://hdl.handle.net/2440/9798
dc.language.isoen
dc.publisherAmer Physiological Soc
dc.source.urihttps://doi.org/10.1152/ajplung.2000.279.6.l1172
dc.subjectBronchi
dc.subjectPulmonary Alveoli
dc.subjectRespiratory Mucosa
dc.subjectTrachea
dc.subjectTumor Cells, Cultured
dc.subjectEpithelial Cells
dc.subjectAnimals
dc.subjectSheep
dc.subjectSwine
dc.subjectHumans
dc.subjectAdenocarcinoma, Bronchiolo-Alveolar
dc.subjectLung Neoplasms
dc.subjectHydrogen Peroxide
dc.subjectZinc
dc.subjectEthylenediamines
dc.subjectButyrates
dc.subjectTosyl Compounds
dc.subjectCoumarins
dc.subjectQuinolones
dc.subjectCaspases
dc.subjectOligopeptides
dc.subjectChelating Agents
dc.subjectFluorescent Dyes
dc.subjectOxidants
dc.subjectMicroscopy, Electron
dc.subjectMicroscopy, Fluorescence
dc.subjectApoptosis
dc.subjectEnzyme Activation
dc.subjectDrug Synergism
dc.subjectCaspase 3
dc.titleVisualization of labile zinc and its role in apoptosis of primary airway epithelial cells and cell lines
dc.typeJournal article
pubs.publication-statusPublished

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