Secretome analysis of virulent Pyrenophora teres f. teres isolates

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dc.contributor.authorIsmail, I.
dc.contributor.authorAble, A.
dc.date.issued2016
dc.descriptionOnline publication September 2016
dc.description.abstractPyrenophora teres f. teres (Ptt) causes net form net blotch disease of barley, partially by producing necrosis-inducing proteins. The protein profiles of the culture filtrates of 28 virulent isolates were compared by a combination of 2DE and 1D-PAGE with 105 spots and 51 bands chosen for analysis by liquid chromatography electrospray ionization tandem mass spectrometry. A total of 259 individual proteins were identified with 63 of these proteins being common to the selected virulent isolates. Ptt secretes a broad spectrum of proteins including cell wall degrading enzymes; virulence factors and effectors; proteins associated with fungal pathogenesis and development; and proteins related to oxidation–reduction processes. Potential virulence factors and effectors identified included proteins with glucosidase activity, ricin B and concanavalin A-like lectins, glucanases, spherulin, cutinase, pectin lyase, leucine-rich repeat protein, and ceratoplatanin. Small proteins with unknown function but cysteine-rich, common to effectors, were also identified. Differences in the secretion profile of the Ptt isolates have also provided important insight into the different mechanisms contributing to virulence and the development of net form net blotch symptoms.
dc.description.statementofresponsibilityIsmail A. Ismail and Amanda J. Able
dc.identifier.citationProteomics, 2016; 16(20):2625-2636
dc.identifier.doi10.1002/pmic.201500498
dc.identifier.issn1615-9861
dc.identifier.issn1615-9861
dc.identifier.orcidAble, A. [0000-0003-1135-6515]
dc.identifier.urihttp://hdl.handle.net/2440/101280
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.rights© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.source.urihttps://doi.org/10.1002/pmic.201500498
dc.subjectBarley net blotch disease; Plant pathogen; Proteinaceous toxins; Systems biology; Virulence
dc.titleSecretome analysis of virulent Pyrenophora teres f. teres isolates
dc.typeJournal article
pubs.publication-statusPublished

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