Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model

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dc.contributor.authorMrozik, K.
dc.contributor.authorWada, N.
dc.contributor.authorMarino, V.
dc.contributor.authorRichter, W.
dc.contributor.authorShi, S.
dc.contributor.authorWheeler, D.
dc.contributor.authorGronthos, S.
dc.contributor.authorBartold, P.
dc.date.issued2013
dc.description.abstractAim: To investigate the capacity of allogeneic periodontal ligament stem cells (PDLSCs) to regenerate periodontal tissues using an ovine periodontal defect model. Materials & methods: Surgically created zero-wall dehiscence periodontal defects created in Merino sheep were filled with 1 × 107 allogeneic PDLSCs attached to Gelfoam®, Gelfoam alone or left untreated. After 4 weeks, histological analysis was performed to assess periodontal regeneration. Results: Allogeneic PDLSCs were well tolerated by recipient animals. The mean area of new alveolar bone was significantly greater in the PDLSC + Gelfoam treatment group compared with the defect-alone group. The PDLSC + Gelfoam and Gelfoam-only treatment groups displayed significantly greater length of new cementum and percentage of cementum regrowth compared with the defect-alone group. New Sharpey‘s fibers were generally more organized and significantly thicker within the PDLSC + Gelfoam treatment group. The PDLSC + Gelfoam treatment group also showed a trend of increased Sharpey‘s fiber attachment length compared with the Gelfoam-only and defect-alone groups. Conclusion: These studies support the potential use of allogeneic PDLSC preparations as viable therapies for periodontal regeneration in the clinical setting.
dc.description.statementofresponsibilityKrzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Ward Richter, Songtao Shi, Donna L Wheeler, Stan Gronthos, and P Mark Bartold
dc.identifier.citationRegenerative Medicine, 2013; 8(6):711-723
dc.identifier.doi10.2217/rme.13.66
dc.identifier.issn1746-0751
dc.identifier.issn1746-076X
dc.identifier.orcidMrozik, K. [0000-0002-4890-8208]
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]
dc.identifier.orcidBartold, P. [0000-0002-5695-3877] [0000-0002-6225-3084]
dc.identifier.urihttp://hdl.handle.net/2440/81470
dc.language.isoen
dc.publisherFuture Medicine Ltd
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/299009
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/453599
dc.rights© Future Medicine
dc.source.urihttps://doi.org/10.2217/rme.13.66
dc.subjectallogeneic
dc.subjectalveolar bone
dc.subjectcementum
dc.subjectperiodontal ligament stem cells
dc.subjectregeneration
dc.subjectSharpey‘s fibers
dc.titleRegeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model
dc.typeJournal article
pubs.publication-statusPublished

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