Transcriptome comparison of Shiraz (Vitis vinifera) grapevines in distinct sub-regions of the Barossa Valley

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dc.contributor.advisorGilliham, Matthew
dc.contributor.advisorRodriguez Lopez, Carlos Marcelino
dc.contributor.authorSai, Na
dc.contributor.schoolSchool of Agriculture, Food and Wineen
dc.date.issued2016
dc.descriptionTitle page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.
dc.description.abstractStudying the interaction between grapevines and the environment may provide insights of how terroir drives unique characters in wine. Analysing changes in gene expression between different environmental conditions provides a first step in understanding genes that may play a role in grapevine adaption. We, therefore, carried out RNA-seq analysis on Shiraz grapevine leaf tissue harvested from two sub-regions of the Barossa Valley to investigate whether gene expression changes occurred in response to two important environmental factors for plant growth, temperature and elevation. Young leaves from three vineyards in the Barossa central ground and three in the Eden Valley were sampled at budburst. The transcriptome profiling of all samples was clustered by vineyard and separated by region. In total, 429 genes showed significant changes in gene expression between two regions (FDR < 0.001). Among the differentially expressed genes, we found a subset of genes enriched in Gene Ontology (GO) terms that are related to environmental response, including abiotic stress and external biotic stress (Q-value < 0.05). Our study provides preliminary analysis of transcriptome changes in different sub-regions of Barossa Valley and identified potential candidate genes involved in adaptive responses under different environmental condition.en
dc.description.dissertationThesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016en
dc.identifier.urihttp://hdl.handle.net/2440/112720
dc.provenanceMasters of Biotechnology (Plant Biotechnology) by courseworken
dc.subjectcourseworken
dc.subjecttranscriptome analysisen
dc.subjectRNA-seqen
dc.subjectgrapevine adaptionen
dc.subjectBarossa Valleyen
dc.subjectterroiren
dc.subjectShirazen
dc.titleTranscriptome comparison of Shiraz (Vitis vinifera) grapevines in distinct sub-regions of the Barossa Valleyen
dc.typeThesesen

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