Expression of NEDD4L and ENaC in Urinary extracellular vesicles in pre-eclampsia
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Date
2023
Authors
Leung, P.Y.M.
Katerelos, M.
Choy, S.
Cook, N.
Lee, M.
Paizis, K.
Abboud, A.
Manning, J.A.
Mount, P.F.
Power, D.A.
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Journal article
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Hypertension in Pregnancy, 2023; 42(1, article no. 2232029):1-9
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Abstract
Objective To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls. Methods Urine was collected from pre-eclamptic women (PE, n = 20) or during normal pregnancy (NP, n = 20). UEV were separated by differential ultracentrifugation. NEDD4L, & alpha;-ENaC and & gamma;-ENaC were identified by immunoblotting. Results There was no difference in the expression of NEDD4L (p = 0.17) and & alpha;-ENaC (p = 0.10). PE subjects showed increased expression of & gamma;-ENaC by 6.9-fold compared to NP (p < 0.0001). Conclusion ENaC expression is upregulated in UEV of pre-eclamptic subjects but was not associated with changes in NEDD4L.
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Data source: supplementary material, https://doi.org/10.1080/10641955.2023.2232029
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Copyright 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. (http://creativecommons.org/licenses/by/4.0/)
Access Condition Notes: This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.