Analysis of plastid DNA-like sequences within the nuclear genomes of higher plants.

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dc.contributor.authorAyliffe, M.
dc.contributor.authorScott, N.
dc.contributor.authorTimmis, J.
dc.date.issued1998
dc.description.abstractA wide-ranging examination of plastid (pt)DNA sequence homologies within higher plant nuclear genomes (promiscuous DNA) was undertaken. Digestion with methylation-sensitive restriction enzymes and Southern analysis was used to distinguish plastid and nuclear DNA in order to assess the extent of variability of promiscuous sequences within and between plant species. Some species, such as Gossypium hirsutum (cotton), Nicotiana tabacum (tobacco), and Chenopodium quinoa, showed homogenity of these sequences, while intraspecific sequence variation was observed among different cultivars of Pisum sativum (pea), Hordeum vulgare (barley), and Triticum aestivum (wheat). Hypervariability of plastid sequence homologies was identified in the nuclear genomes of Spinacea oleracea (spinach) and Beta vulgaris (beet), in which individual plants were shown to possess a unique spectrum of nuclear sequences with ptDNA homology. This hypervariability apparently extended to somatic variation in B. vulgaris. No sequences with ptDNA homology were identified by this method in the nuclear genome of Arabidopsis thaliana.
dc.identifier.citationMolecular Biology and Evolution (MBE), 1998; 15(6):738-745
dc.identifier.doi10.1093/oxfordjournals.molbev.a025977
dc.identifier.issn0737-4038
dc.identifier.issn1537-1719
dc.identifier.urihttp://hdl.handle.net/2440/11480
dc.language.isoen
dc.publisherSOC MOLECULAR BIOLOGY EVOLUTION
dc.source.urihttps://doi.org/10.1093/oxfordjournals.molbev.a025977
dc.subjectPlastids
dc.subjectPlants
dc.subjectArabidopsis
dc.subjectChenopodiaceae
dc.subjectSpinacia oleracea
dc.subjectGossypium
dc.subjectHordeum
dc.subjectTriticum
dc.subjectPlants, Toxic
dc.subjectDNA, Plant
dc.subjectSpecies Specificity
dc.subjectSequence Homology, Nucleic Acid
dc.subjectGenome, Plant
dc.subjectEdible Grain
dc.subjectNicotiana
dc.subjectPisum sativum
dc.titleAnalysis of plastid DNA-like sequences within the nuclear genomes of higher plants.
dc.typeJournal article
pubs.publication-statusPublished

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