Identification of consensus head and neck cancer-associated microbiota signatures: a systematic review and meta-analysis of 16S rRNA and The Cancer Microbiome Atlas datasets

Simple item page
dc.contributor.authorYeo, K.
dc.contributor.authorLi, R.
dc.contributor.authorWu, F.
dc.contributor.authorBouras, G.
dc.contributor.authorMai, L.T.H.
dc.contributor.authorSmith, E.
dc.contributor.authorWormald, P.-J.
dc.contributor.authorValentine, R.
dc.contributor.authorPsaltis, A.J.
dc.contributor.authorVreugde, S.
dc.contributor.authorFenix, K.
dc.date.issued2024
dc.descriptionPublished 01 February 2024
dc.description.abstractIntroduction. Multiple reports have attempted to describe the tumour microbiota in head and neck cancer (HNSC).Gap statement. However, these have failed to produce a consistent microbiota signature, which may undermine understanding the importance of bacterial-mediated effects in HNSC.Aim. The aim of this study is to consolidate these datasets and identify a consensus microbiota signature in HNSC.Methodology. We analysed 12 published HNSC 16S rRNA microbial datasets collected from cancer, cancer-adjacent and non-cancer tissues to generate a consensus microbiota signature. These signatures were then validated using The Cancer Microbiome Atlas (TCMA) database and correlated with the tumour microenvironment phenotypes and patient's clinical outcome.Results. We identified a consensus microbial signature at the genus level to differentiate between HNSC sample types, with cancer and cancer-adjacent tissues sharing more similarity than non-cancer tissues. Univariate analysis on 16S rRNA datasets identified significant differences in the abundance of 34 bacterial genera among the tissue types. Paired cancer and cancer-adjacent tissue analyses in 16S rRNA and TCMA datasets identified increased abundance in Fusobacterium in cancer tissues and decreased abundance of Atopobium, Rothia and Actinomyces in cancer-adjacent tissues. Furthermore, these bacteria were associated with different tumour microenvironment phenotypes. Notably, high Fusobacterium signature was associated with high neutrophil (r=0.37, P<0.0001), angiogenesis (r=0.38, P<0.0001) and granulocyte signatures (r=0.38, P<0.0001) and better overall patient survival [continuous: HR 0.8482, 95 % confidence interval (CI) 0.7758-0.9273, P=0.0003].Conclusion. Our meta-analysis demonstrates a consensus microbiota signature for HNSC, highlighting its potential importance in this disease.
dc.description.statementofresponsibilityKenny Yeo, Runhao Li, Fangmeinuo Wu, George Bouras, Linh T.H. Mai, Eric Smith, Peter-John Wormald, Rowan Valentine, Alkis James Psaltis, Sarah Vreugde, and Kevin Fenix
dc.identifier.citationJournal of Medical Microbiology, 2024; 73(2):001799-1-001799-18
dc.identifier.doi10.1099/jmm.0.001799
dc.identifier.issn0022-2615
dc.identifier.issn1473-5644
dc.identifier.orcidYeo, K. [0000-0001-5733-0463]
dc.identifier.orcidLi, R. [0000-0001-5577-049X]
dc.identifier.orcidBouras, G. [0000-0002-5885-4186]
dc.identifier.orcidSmith, E. [0000-0003-2958-3492]
dc.identifier.orcidWormald, P.-J. [0000-0001-7753-7277]
dc.identifier.orcidPsaltis, A.J. [0000-0003-2197-0797] [0000-0003-2967-1855]
dc.identifier.orcidVreugde, S. [0000-0003-4719-9785]
dc.identifier.orcidFenix, K. [0000-0003-1619-1406]
dc.identifier.urihttps://hdl.handle.net/2440/140423
dc.language.isoen
dc.publisherMicrobiology Society
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1196832
dc.rights2024 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
dc.source.urihttp://dx.doi.org/10.1099/jmm.0.001799
dc.subjecttumour microbiota; head and neck cancer; 16S rRNA sequencing; meta-analysis
dc.subject.meshHumans
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshConsensus
dc.subject.meshHead and Neck Neoplasms
dc.subject.meshMicrobiota
dc.subject.meshBacteria
dc.subject.meshTumor Microenvironment
dc.titleIdentification of consensus head and neck cancer-associated microbiota signatures: a systematic review and meta-analysis of 16S rRNA and The Cancer Microbiome Atlas datasets
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_140423.pdf
Size:
6.65 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Surgery publications