Impact of embryo number and periconceptional undernutrition on factors regulating adipogenesis, lipogenesis, and metabolism in adipose tissue in the sheep fetus

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dc.contributor.authorLie, S.
dc.contributor.authorMorrison, J.
dc.contributor.authorWyss, O.
dc.contributor.authorOzanne, S.
dc.contributor.authorZhang, S.
dc.contributor.authorWalker, S.
dc.contributor.authorKleemann, D.
dc.contributor.authorMacLaughlin, S.
dc.contributor.authorRoberts, C.
dc.contributor.authorMcMillen, I.
dc.date.issued2013
dc.description.abstractMaternal undernutrition around the time of conception is associated with an increased risk of insulin resistance in adulthood. We hypothesized that maternal undernutrition during the periconceptional (PCUN: -60 to 7 days) and/or preimplantation (PIUN: 0-7 days) periods would result in a decrease in UCP1 expression and the abundance of insulin signaling molecules and an increase in the abundance of factors that regulate adipogenesis and lipogenesis in fetal perirenal adipose tissue (PAT) and that these effects would be different in singletons and twins. Maternal PCUN and PIUN resulted in a decrease in UCP1 expression in PAT, and PIUN resulted in higher circulating insulin concentrations, an increased abundance of pPKCζ and PDK4, and a decreased abundance of Akt1, phosphorylated mTOR, and PPARγ in PAT in singleton and twin fetuses. In singletons, there was also a decrease in the abundance of p110β in PAT in the PCUN and PIUN groups and an increase in total AMPKα in PAT in the PIUN group. In twins, however, there was an increase in the abundance of mTOR in the PCUN group and an increase in PDK2 and decrease in total AMPKα in the PIUN group. Thus exposure to periconceptional undernutrition programs changes in the thermogenic capacity and the insulin and fatty acid oxidation signaling pathway in visceral fat, and these effects are different in singletons and twins. These findings are important, as the thermogenic capacity of brown fat and the insulin sensitivity of visceral fat are important determinants of the risk of developing obesity and an insulin resistance phenotype in later life.
dc.description.statementofresponsibilityShervi Lie, Janna L. Morrison, Olivia Williams-Wyss, Susan E. Ozanne, Song Zhang, Simon K. Walker, David O. Kleemann, Severence M. MacLaughlin, Claire T. Roberts, and I. Caroline McMillen
dc.identifier.citationAmerican Journal of Physiology: Endocrinology and Metabolism, 2013; 305(8):931-941
dc.identifier.doi10.1152/ajpendo.00180.2013
dc.identifier.issn0193-1849
dc.identifier.issn1522-1555
dc.identifier.orcidRoberts, C. [0000-0002-9250-2192]
dc.identifier.urihttp://hdl.handle.net/2440/81105
dc.language.isoen
dc.publisherAmerican Physiological Society
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1020749
dc.rightsCopyright © 2013 the American Physiological Society
dc.source.urihttps://doi.org/10.1152/ajpendo.00180.2013
dc.subjectpregnancy
dc.subjectnutrition
dc.subjectprogramming
dc.subjectmetabolism
dc.titleImpact of embryo number and periconceptional undernutrition on factors regulating adipogenesis, lipogenesis, and metabolism in adipose tissue in the sheep fetus
dc.typeJournal article
pubs.publication-statusPublished

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