Renal transplantation reverses functional deficiencies in circulating dendritic cell subsets in chronic renal failure patients

Simple item page
dc.contributor.authorLim, W.
dc.contributor.authorKireta, S.
dc.contributor.authorThomson, A.
dc.contributor.authorRuss, G.
dc.contributor.authorCoates, P.
dc.date.issued2006
dc.description.abstract<h4>Background</h4>Dendritic cell (DC) subsets play critical roles in regulation of innate and adaptive immune responses. These important antigen-presenting cells have not been extensively analyzed in chronic renal failure (CRF), during dialysis, or before and after renal transplantation.<h4>Methods</h4>The incidence of circulating precursor (pre)-DC subsets relative to total peripheral blood mononuclear cells was analyzed in healthy controls, haemodialysis patients, peritoneal dialysis patients, CRF patients, and renal transplant (RT) recipients. DC subsets were identified and characterized phenotypically by multicolour flow cytometric analysis and purified by immunomagnetic bead isolation respectively. Cytokine production and circulating DC mobilizing cytokines were determined by ELISA.<h4>Results</h4>The incidence of circulating prePDC was reduced in all patients, but the incidence of circulating preMDC was comparable in RT and dialysis patients compared to healthy controls. CRF patients exhibited the lowest incidence of circulating preMDC and prePDC. Immunomagnetic bead-isolated preMDC and prePDC from haemodialysis patients were functionally impaired (reduced expression of surface costimulatory molecules and interleukin-12p70 production following bacterial lipopolysaccharide stimulation, and reduced interferon-alpha production following herpes simplex virus stimulation respectively, compared to healthy controls and RT recipients. Glomerular filtration rate correlated significantly with the incidence of circulating preMDC, but not prePDC.<h4>Conclusions</h4>Deficiencies in the incidence and function of precursor DC can be reversed with successful renal transplantation achieving normal renal function. However, the finding of reduced incidence of circulating prePDC in the peripheral blood in RT recipients may be of significance in the pathogenesis of infections and malignancies.
dc.identifier.citationTransplantation, 2006; 81(2):160-168
dc.identifier.doi10.1097/01.tp.0000188620.72969.56
dc.identifier.issn0041-1337
dc.identifier.issn1534-6080
dc.identifier.urihttp://hdl.handle.net/2440/23484
dc.language.isoen
dc.publisherLippincott Williams & Wilkins
dc.source.urihttps://doi.org/10.1097/01.tp.0000188620.72969.56
dc.subjectDendritic Cells
dc.subjectHumans
dc.subjectKidney Failure, Chronic
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor
dc.subjectMembrane Proteins
dc.subjectImmunosuppressive Agents
dc.subjectGlomerular Filtration Rate
dc.subjectPeritoneal Dialysis, Continuous Ambulatory
dc.subjectRenal Dialysis
dc.subjectKidney Transplantation
dc.subjectCase-Control Studies
dc.subjectProspective Studies
dc.subjectAdult
dc.subjectAged
dc.subjectMiddle Aged
dc.subjectFemale
dc.subjectMale
dc.subjectCalcineurin Inhibitors
dc.titleRenal transplantation reverses functional deficiencies in circulating dendritic cell subsets in chronic renal failure patients
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Medicine publications