Converse Smith-Martin cell cycle kinetics by transformed B lymphocytes

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dc.contributor.authorPham, K.
dc.contributor.authorKan, A.
dc.contributor.authorWhitehead, L.
dc.contributor.authorHennessy, R.J.
dc.contributor.authorRogers, K.
dc.contributor.authorHodgkin, P.D.
dc.date.issued2018
dc.description.abstractRecent studies using direct live cell imaging have reported that individual B lymphocytes have correlated transit times between their G1 and S/G2/M phases. This finding is in contradiction with the influential model of Smith and Martin that assumed the bulk of the total cell cycle time variation arises in the G1 phase of the cell cycle with little contributed by the S/G2/M phase. Here we extend these studies to examine the relation between cell cycle phase lengths in two B lymphoma cell lines. We report that transformed B lymphoma cells undergo a short G1 period that displays little correlation with the time taken for the subsequent S/G2/M phase. Consequently, the bulk of the variation noted for total division times within a population is found in the S/G2/M phases and not the G1 phase. Models that reverse the expected source of variation and assume a single deterministic time in G1 followed by a lag + exponential distribution for S/G2/M fit the data well. These models can be improved further by adopting two sequential distributions or by using the stretched lognormal model developed for primary lymphocytes. We propose that shortening of G1 transit times and uncoupling from other cell cycle phases may be a hallmark of lymphocyte transformation that could serve as an observable phenotypic marker of cancer evolution.
dc.description.statementofresponsibilityK. Pham, A. Kan, L. Whitehead, R. J. Hennessy, K. Rogers, P. D. Hodgkin
dc.identifier.citationCell Cycle, 2018; 17(16):2041-2051
dc.identifier.doi10.1080/15384101.2018.1511511
dc.identifier.issn1538-4101
dc.identifier.issn1551-4005
dc.identifier.orcidKan, A. [0000-0002-2432-0047]
dc.identifier.urihttps://hdl.handle.net/2440/132713
dc.language.isoen
dc.publisherInforma UK
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1057831
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1054925
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/361646
dc.rights© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
dc.source.urihttps://doi.org/10.1080/15384101.2018.1511511
dc.subjectCell cycle; Smith-Martin model; G1; S/G2/M; FUCCI; cancer
dc.subject.meshB-Lymphocytes
dc.subject.meshCell Line, Transformed
dc.subject.meshCell Line, Tumor
dc.subject.meshClone Cells
dc.subject.meshAnimals
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshCell Cycle
dc.subject.meshG1 Phase
dc.subject.meshKinetics
dc.subject.meshFluorescence
dc.subject.meshModels, Biological
dc.subject.meshUbiquitination
dc.titleConverse Smith-Martin cell cycle kinetics by transformed B lymphocytes
dc.typeJournal article
pubs.publication-statusPublished

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