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Ectopic bone formation by mesenchymal stem cells derived from human term placenta and the decidua

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dc.contributor.authorKusuma, G.
dc.contributor.authorMenicanin, D.
dc.contributor.authorGronthos, S.
dc.contributor.authorManuelpillai, U.
dc.contributor.authorAbumaree, M.
dc.contributor.authorPertile, M.
dc.contributor.authorBrennecke, S.
dc.contributor.authorKalionis, B.
dc.contributor.editorMatsusaki, M.
dc.date.issued2015
dc.description.abstractMesenchymal stem cells (MSCs) are one of the most attractive cell types for cell-based bone tissue repair applications. Fetal-derived MSCs and maternal-derived MSCs have been isolated from chorionic villi of human term placenta and the decidua basalis attached to the placenta following delivery, respectively. Chorionic-derived MSCs (CMSCs) and decidua-derived MSCs (DMSCs) generated in this study met the MSCs criteria set by International Society of Cellular Therapy. These criteria include: (i) adherence to plastic; (ii) >90% expression of CD73, CD105, CD90, CD146, CD44 and CD166 combined with <5% expression of CD45, CD19 and HLA-DR; and (iii) ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. In vivo subcutaneous implantation into SCID mice showed that both bromo-deoxyuridine (BrdU)-labelled CMSCs and DMSCs when implanted together with hydroxyapatite/tricalcium phosphate particles were capable of forming ectopic bone at 8-weeks post-transplantation. Histological assessment showed expression of bone markers, osteopontin (OPN), osteocalcin (OCN), biglycan (BGN), bone sialoprotein (BSP), and also a marker of vasculature, alpha-smooth muscle actin (α-SMA). This study provides evidence to support CMSCs and DMSCs as cellular candidates with potent bone forming capacity.
dc.description.statementofresponsibilityGina D. Kusuma, Danijela Menicanin, Stan Gronthos, Ursula Manuelpillai, Mohamed H. Abumaree, Mark D. Pertile, Shaun P. Brennecke, Bill Kalionis
dc.identifier.citationPLoS ONE, 2015; 10(10):e0141246-1-e0141246-13
dc.identifier.doi10.1371/journal.pone.0141246
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.orcidMenicanin, D. [0000-0002-1178-2293]
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]
dc.identifier.urihttp://hdl.handle.net/2440/96972
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights© 2015 Kusuma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.source.urihttps://doi.org/10.1371/journal.pone.0141246
dc.subjectDecidua
dc.subjectCells, Cultured
dc.subjectMesenchymal Stem Cells
dc.subjectPlacenta
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectMice, SCID
dc.subjectMesenchymal Stem Cell Transplantation
dc.subjectFlow Cytometry
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectCell Differentiation
dc.subjectOsteogenesis
dc.subjectPregnancy
dc.subjectFemale
dc.subjectBiomarkers
dc.titleEctopic bone formation by mesenchymal stem cells derived from human term placenta and the decidua
dc.typeJournal article
pubs.publication-statusPublished

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