Stability of serotonin-selective antidepressants in sterile and decomposing liver tissue

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dc.contributor.authorButzbach, D.
dc.contributor.authorStockham, P.
dc.contributor.authorKobus, H.
dc.contributor.authorSims, N.
dc.contributor.authorByard, R.
dc.contributor.authorLokan, R.
dc.contributor.authorWalker, S.
dc.date.issued2013
dc.description.abstractIt is well established that bacteria are capable of degrading selected drugs during decomposition. The aim of this study was to investigate the stability of several serotonin-selective reuptake inhibitor antidepressants and venlafaxine during putrefaction in porcine liver macerate inoculated with porcine cecal contents rich in bacteria. Blank liver matrices, sterile liver macerates, and sterile aqueous controls were included with the experiment performed for 57 days at 20°C under anaerobic conditions. A liquid chromatography/mass spectrometry method was developed for quantitative determination of the drugs investigated in both sterile and decomposed liver matrices. The method was found to encounter matrix effects not detected during the validation stage. Citalopram, paroxetine, sertraline, venlafaxine, and fluoxetine were found to be stable under the experimental conditions; however, fluvoxamine was found to be decreased by c. 50% over 57 days in bacterially inoculated liver macerate. This study suggests that fluvoxamine concentrations in cases with evidence of decomposition/putrefaction should be interpreted with extra caution.
dc.description.statementofresponsibilityDanielle M. Butzbach, Peter C. Stockham, Hilton J. Kobus, Noel Sims, Roger W. Byard, Robert J. Lokan, and G. Stewart Walker
dc.identifier.citationJournal of Forensic Sciences, 2013; 58(SUPPL. 1):117-125
dc.identifier.doi10.1111/j.1556-4029.2012.02266.x
dc.identifier.issn0022-1198
dc.identifier.issn1556-4029
dc.identifier.orcidByard, R. [0000-0002-0524-5942]
dc.identifier.urihttp://hdl.handle.net/2440/76053
dc.language.isoen
dc.publisherAmer Soc Testing Materials
dc.rights© 2012 American Academy of Forensic Sciences
dc.source.urihttps://doi.org/10.1111/j.1556-4029.2012.02266.x
dc.subjectforensic science
dc.subjectforensic toxicology
dc.subjectpostmortem
dc.subjectstability
dc.subjectliver
dc.subjectantidepressant
dc.subjectputrefaction
dc.subjectdecomposition
dc.subjectmass spectrometry
dc.titleStability of serotonin-selective antidepressants in sterile and decomposing liver tissue
dc.typeJournal article
pubs.publication-statusPublished

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