Naturally occurring Sarcocystis neurona-like infection in a dog with myositis
Date
2005
Authors
Vashisht, K.
Lichtensteigera, C.
Miller, L.
Gondim, L.
McAllister, M.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Veterinary Parasitology, 2005; 133(1):19-25
Statement of Responsibility
Kapil Vashisht, Carol A. Lichtensteiger, Lou A. Miller, Luis F.P. Gondim, Milton M. McAllister
Conference Name
Abstract
Tissue stages similar to those of Sarcocystis neurona, the causative agent of equine protozoal myeloencephalitis, were identified in skeletal muscles of a dog. The dog, a 6-year-old Labrador retriever, was seropositive for Toxoplasma gondii infection and euthanized due to a history of polymyositis and progressive muscular atrophy. Histologically, 30, variably sized, microscopic, intracellular sarcocysts were observed in 60 sections of skeletal muscles taken from the neck, fore limbs and hind limbs. The cysts were only observed in inflamed skeletal muscles, but were mostly in myocytes at the periphery of areas infiltrated with leukocytes. Ultrastructurally, the cyst wall had villar protrusions consistent with sarcocysts. Immunohistochemistry with monoclonal S. neurona antibodies demonstrated positive labeling of zoites in merozoites or schizonts in the skeletal muscle interstitium, but no labeling of the sarcocysts. Initial PCR analysis with primers amplifying a genetic sequence encoding Apicomplexan 18s rRNA, and subsequent PCR analysis with differentiating primers indicated that the genetic sequences had 100% identity with sequences reported for S. neurona.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2005 Elsevier B.V. All rights reserved.