Comparison of loop-mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis

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dc.contributor.authorGelaw, B.
dc.contributor.authorShiferaw, Y.
dc.contributor.authorMenberu, M.
dc.contributor.authorBashaw, A.A.
dc.date.issued2017
dc.descriptionPublished online: 17 January 2017
dc.description.abstractBackground: Tuberculosis (TB) caused by Mycobacterium tuberculosis is one of the leading causes of death from infectious diseases worldwide. Sputum smear microscopy remains the most widely available pulmonary TB diagnostic tool particularly in resource limited settings. A highly sensitive diagnostic with minimal infrastructure, cost and training is required. Hence, we assessed the diagnostic performance of Loop-mediated isothermal amplification (LAMP) assay in detecting M.tuberculosis infection in sputum sample compared to LED fluorescent smear microscopy and culture. Method: A cross-sectional study was conducted at the University of Gondar Hospital from June 01, 2015 to August 30, 2015. Pulmonary TB diagnosis using sputum LED fluorescence smear microscopy, TB-LAMP assay and culture were done. A descriptive analysis was used to determine demographic characteristics of the study participants. Analysis of sensitivity and specificity for smear microscopy and TB-LAMP compared with culture as a reference test was performed. Cohen’s kappa was calculated as a measure of agreement between the tests. Results: A total of 78 pulmonary presumptive TB patients sputum sample were analyzed. The overall sensitivity and specificity of LAMP were 75 and 98%, respectively. Among smear negative sputum samples, 33.3% sensitivity and 100% specificity of LAMP were observed. Smear microscopy showed 78.6% sensitivity and 98% specificity. LAMP and smear in series had sensitivity of 67.8% and specificity of 100%. LAMP and smear in parallel had sensitivity of 85.7% and specificity of 96%. The agreement between LAMP and fluorescent smear microscopy tests was very good (κ = 0.83, P-value ≤0.0001). Conclusions: TB-LAMP showed similar specificity but a slightly lower sensitivity with LED fluorescence microscopy. The specificity of LAMP and smear smear microscopy in series was high. The sensitivity of LAMP was insufficient for smear negative sputum samples.
dc.description.statementofresponsibilityBaye Gelaw, Yitayal Shiferaw, Marta Alemayehu and Abate Assefa Bashaw
dc.identifier.citationBMC Infectious Diseases, 2017; 17(1):79-1-79-6
dc.identifier.doi10.1186/s12879-016-2140-8
dc.identifier.issn1471-2334
dc.identifier.issn1471-2334
dc.identifier.orcidMenberu, M. [0000-0002-0696-5140]
dc.identifier.urihttp://hdl.handle.net/2440/124162
dc.language.isoen
dc.publisherBioMed Central
dc.rights© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.source.urihttps://doi.org/10.1186/s12879-016-2140-8
dc.subjectLoop-mediated isothermal amplification; Tuberculosi;, Smear microscopy; Culture
dc.titleComparison of loop-mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis
dc.typeJournal article
pubs.publication-statusPublished

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