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A new pineoblastoma cell line, PER-480, with der(10)t(10;17), der(16)t(1;16), & enhanced MYC expression in the absence of gene amplification.

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dc.contributor.authorKees, U.
dc.contributor.authorSpagnolo, D.
dc.contributor.authorHallam, L.
dc.contributor.authorFord, J.
dc.contributor.authorRanford, P.
dc.contributor.authorBaker, D.
dc.contributor.authorCallen, D.
dc.contributor.authorBiegel, J.
dc.date.issued1998
dc.description.abstractPineoblastoma is a rare, but highly malignant tumor of the central nervous system (CNS) in children and is classified as a central primitive neuroectodermal tumor (PNET). Despite notable recent advances in understanding the molecular genetic basis of malignancies, the pathogenesis of PNETs remains enigmatic. There is scant information on the cytogenetics of PNETs arising in the pineal gland and the only three reported cases did not show any common aberrations. Here we report the establishment and characterization of a new pineoblastoma cell line, PER-480. The biopsy material and the cell line were characterized using light and electron microscopy and immunohistochemical analyses. The cell line was examined for expression of cell surface markers using a panel of monoclonal antibodies and by cytogenetic analysis. MYC family genes were studied at the DNA, RNA, and protein level. Cell line PER-480 showed neuronal differentiation and the karyotype demonstrated two abnormalities, a der(10)t(10;17) and a der(16)t(1;16). An intriguing finding is that all three pineoblastoma cell lines established in our laboratory, PER-452, PER-453, and PER-480, showed enhanced expression but not amplification of a member of the MYC family of proto-oncogenes. Cell line PER-480 reported here will be useful for the further investigation of the molecular genetic basis of central PNETs.
dc.description.statementofresponsibilityUrsula R. Kees, Dominic Spagnolo, Lavinia A. Hallam, Jette Ford, Pamela R. Ranford, David L. Baker, David F. Callen, and Jaclyn A. Biegel
dc.identifier.citationCancer Genetics, 1998; 100(2):159-164
dc.identifier.doi10.1016/S0165-4608(97)00030-7
dc.identifier.issn0165-4608
dc.identifier.issn1873-4456
dc.identifier.orcidCallen, D. [0000-0002-6189-9991]
dc.identifier.urihttp://hdl.handle.net/2440/11400
dc.language.isoen
dc.publisherELSEVIER SCIENCE INC
dc.rights© Elsevier Science Inc., 1998
dc.source.urihttps://doi.org/10.1016/s0165-4608(97)00030-7
dc.subjectPineal Gland
dc.subjectTumor Cells, Cultured
dc.subjectChromosomes, Human
dc.subjectHumans
dc.subjectPinealoma
dc.subjectBrain Neoplasms
dc.subjectTranslocation, Genetic
dc.subjectKaryotyping
dc.subjectGene Amplification
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectGenes, myc
dc.subjectInfant
dc.subjectMale
dc.titleA new pineoblastoma cell line, PER-480, with der(10)t(10;17), der(16)t(1;16), & enhanced MYC expression in the absence of gene amplification.
dc.typeJournal article
pubs.publication-statusPublished

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